antibody

Sheep anti-human Vimentin, Whole IgG from antiserum

MBS512168

10 mg

280 USD

polyclonal

sheep

nonconjugated

Antibody

Sheep anti-human Vimentin, Whole IgG from antiserum

Vimentin

Vimentin, human

vimentin; Vimentin; vimentin; OTTHUMP00000019224; OTTHUMP00000019225; vimentin

VIM; VIM; FLJ36605

VIME_HUMAN

Polyclonal

Sheep

This antibody is specific for vimentin as demonstrated by immunoelectrophoresis and ELISA.

Purified IgG. Vial containing ml of whole IgG representing approximately 1 ml of antiserum. Total protein is 10 mg.

Whole IgG, clear liquid.

IgG concentration is mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 13.4.

Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use.

Suitable for use as a source of antibodies to vimentin.

Immunogen: Purified recombinant human vimentin produced in bacteria. This protein constitutes a soluble 20 kDa fragment from the amino-terminus of vimentin.

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.

Intermediate filaments (IFs), along with microfilaments and microtubules, are the three major filament systems that form the architectural basis of most eukaryotic cells. Vimentin, with a molecular weight of 58 kDa, is a Type III intermediate filament found in cells of mesenchymal origin including endothelial cells, megakaryocytes and platelets as well as in most cultured cell lines. As with other IFs, vimentin provides a physical linkage between the plasma membrane and the nuclear envelope. The carboxy-terminus of vimentin associates with the lamin B of the nuclear envelope whereas the amino-terminus associates with the plasma membrane either directly or indirectly. In general, it is believed that IFs, including vimentin, provide a network onto which various enzyme systems, intracellular structures and organelles are spatially arranged within the cytoplasm. IFs may also play an active role in the re-organization of intracellular components in response to extracellular signals via their disassembly and reassembly. In normal vascular endothelium, vimentin, along with other cytoskeletal proteins, are typically not exposed to extracellular plasma proteins. However, with damage to endothelial cells, exposure of these cytoskeletal components may play a role in thrombogenesis or inflammatory responses. For example, it has been demonstrated that vimentin can bind complement components as wells as immunoglobulins. Vimetin has also been shown to bind vitronectin and vitronectin/PAI-1 complexes in LPS-damaged endothelial cells.1-3

1. Steinert, P.M. and Roop, D.R. Molecular and cellular biology of intermediate filaments. Ann. Rev. Biochem. 57:593-625, 1988. 2. Albrect, D.L., Mills, J.W. and Noelle, R.J. Membrane Ig-Cytoskeletal interatctions: receptor cross-linking results in the formation of extensive filamentous arrays of vimentin. J. Immunol. 144:3251-3256, 1990. 3. Podor, T.J. and Loskutoff, D.J. Binding of PAI-1/vitronectin complexes to the intermediate filament cytoskeleton of endotoxin injured endothelial cells. Fibrinolysis (suppl.), 4:263a, 1990.

62414289

NP_003371.2

NM_003380.2

P08670

53521 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Muscle Contraction Pathway (106261); Striated Muscle Contraction Pathway (106262); Striated Muscle Contraction Pathway (198903)

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Subunit structure: Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM. Interacts (via rod region) with PLEC (via CH 1 domain). By similarity. Interacts with SLC6A4. Ref.22 Ref.35. Tissue specificity: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. Ref.12 Ref.17. Post-translational modification: Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. By similarity. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Sequence similarities: Belongs to the intermediate filament family. Sequence caution: The sequence BAB71275.1 differs from that shown. Reason: Intron retention.

10 mg

280 USD