antibody

Sheep anti-human Antithrombin (ATIII), Peroxidase Conjugated Affinity-Purified IgG

MBS512017

0.1 mg

330 USD

polyclonal

sheep

HRP

Antibody

Sheep anti-human Antithrombin (ATIII), Peroxidase Conjugated Affinity-Purified IgG

ATIII - Antithrombin

ATIII - Antithrombin, human

antithrombin III; Antithrombin-III; antithrombin-III; serpin C1; anti-thrombin 3; serpin peptidase inhibitor, clade C, member 1; serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1; serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1; Serpin C1

Serpinc1; Serpinc1; At3; At-3; ATIII; AI114908; At3

ANT3_MOUSE

Polyclonal

Sheep

Prior to conjugation this antibody was specific for antithrombin as demonstrated by immunoelectrophoresis and ELISA.

Affinity purified, peroxidase conj. IgG. Vial containing ml of affinity-purified IgG conjugated to horseradish peroxidase (HRP) through carbohydrate groups. Total protein is 0.1 mg.

APIgG-HRP conjugate as a clear, slightly red-brown liquid.

APIgG-HRP concentration is 1 mg/ml, determined by absorbance using an extinction coefficient (E1%280) of 14. Vial containing 0.1 ml of affinity-purified IgG conjugated to horseradish peroxidase (HRP) through carbonhydrate groups. Total protein is 0

Store between -10 and -20 degree C. Product will become viscous but will not freeze. Avoid storage in frost-free freezers. Keep vial tightly capped. Allow product to warm to room temperature and gently mix before use. Avoid exposure to sodium azide as this is an inhibitor of peroxidase activity.

Suitable as a source of peroxidase-labeled antibodies to ATIII.

Immunogen: Human antithrombin purified from plasma.

Buffer: A buffered stabilizer solution containing 50% (v/v) glycerol. RZ Ratio: (Reinheitszahl, A403/A280): 0.422 as determined spectrophotometrically.

Antithrombin, also known as Antithrombin III (ATIII), is a member of the SERPIN family of proteinase inhibitors and the primary inhibitor of thrombin in plasma. It is produced in the liver and circulates in plasma at ~200 ug/ml (~3.5 uM). Antithrombin inhibits a broad spectrum of serine proteases including thrombin, activated forms of factor X, factor IX, factor XI, factor XII, as well as kallikrein, plasmin and urokinase. Enzyme inhibition by antithrombin occurs through proteolytic cleavage at Arg385-Ser386 and subsequent rapid formation of a stable, inactive 1:1 enzyme-antithrombin complex. Heparin has a profound accelerating effect on the inhibitory activity of antithrombin towards some enzymes. For example, the rate of inhibition of thrombin and activated factor X is increased 1000-fold in the presence of optimal concentrations of heparin, whereas heparin has relatively little effect on the inhibition rate of activated factor XI, activated factor XII and kallikrein. Antithrombin is a single chain molecule with a molecular weight of 59 kDa. Interaction with thrombin results in an SDS-stable thrombin-antithrombin complex of 96 kDa1-3.

1. Damus PS, Rosenberg RD; Antithrombin - Heparin Cofactor; Methods in Enzymology 45, pp 653-669, 1976. 2. Harpel PC; Blood Proteolytic Enzyme Inhibitors: Their Role in Modulating Blood Coagulation and Fibrinolytic Enzyme Pathways; in Hemostasis and Thrombosis, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp. 738-747, J.B. Lippincott Co., Philadelphia PA, USA, 1982. 3. Griffith MJ, Noyes CM, Church FC; Reactive Site Peptide Structural Similarity between Heparin Cofactor II and Antithrombin III; JBC:260, pp 2218-2225, 1985.

258959

P32261

52,004 Da

Common Pathway (366329); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (366326); Hemostasis Pathway (366292)

Function: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin. Subcellular location: Secreted extracellular space. Tissue specificity: Plasma. Post-translational modification: Phosphorylation sites are present in the extracelllular medium. Sequence similarities: Belongs to the serpin family.

0.1 mg

330 USD