product summary
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company name :
MyBioSource
product type :
protein
product name :
Recombinant chimeric antigen CFP10:ESAT6 for Mycobacterium tuberculosis (Koch's bacillus)
catalog :
MBS505078
quantity :
1 mg
price :
915 USD
more info or order :
image
image 1 :
SDS-PAGE analysis (15%) of 5 ul of recombinant CFP10: ESAT6. Purity is approx. 95% as determined by gel electrophoresis. The band which appears just below, corresponds to a in vivo N-terminus or C-terminus degradation product of this same protein, as it is showed in a western blot performed with a his-tag monoclonal antibody.
product information
catalog number :
MBS505078
products type :
Recombinant Protein
products full name :
Recombinant chimeric antigen CFP10:ESAT6 for Mycobacterium tuberculosis (Koch's bacillus)
products short name :
[CFP10:ESAT6]
products name syn :
[ESAT6:CFP10; Chimeric antigen]
other names :
[CFP10, partial]
products gene name :
[CFP10:ESAT6]
sequence length :
100
form :
Dry powder (lyophilized)
concentration :
2.12mg/ml
storage stability :
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 4 degree C to -20 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
app notes :
Not tested. Where this product has not been tested for use in a particular technique,this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates.
image1 heading :
SDS-PAGE
other info1 :
Source: Escherichia coli. Recombinant Antigen: M.tuberculosis chimeric antigen CFP10:ESAT6 (Bekmurzayevaet al., 2013). Batch composition: Components: . his-CFP10:ESAT6. Composition:. Recombinant chimeric antigen with a his-tag in its N-terminus. Absence of precipitation after a freezing and thawing cycle.: Ok. Quality Control: PROTEIN CONCENTRATION DETERMINED ESPECTROPHOTOMETRICALLY. Do 280 =240. A 0.1% (1 g/l)+1.13. Concentration: 2.12mg/mL. The meassurement of the protein concentration has been perfomed with the theoretical extinction coefficient of the recombinant protein obtained from Gill and vonHippel, 1989.
other info2 :
Total Quantity per Aliquot: 1 mg. Observations: Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25ºC without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface. Reconstitution: For lyophilized proteins: with 0.410 ml of sterile double-distilled water (lot specific) for a final concentration of 2.12 mg/ml (lot specific). The protein will be at pH 8.
products categories :
Bacteria
products description :
Mycobacterium tuberculosis genes for the 10 kDa culture filtrate antigen EsxB (CFP10)and the ESAT6 protein have been expressed as a recombinant chimeric antigen fused to a his-tag in its N-terminus.
ncbi gi num :
597960710
ncbi acc num :
AHN50412.1
ncbi mol weight :
Determined by SDS-PAGE, the protein band is between molecular markers of 25,000 and 18,400 Da, while relative molecular mass calculated from amino acid sequence is 22,100.0 Da.
size1 :
1 mg
price1 :
915 USD
size2 :
2 mg
price2 :
1635
size3 :
5 mg
price3 :
3600
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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