catalog number :
MBS505076
products type :
Recombinant Protein
products full name :
Recombinant antigen OMP for Salmonella typhi
products short name :
[OMP]
other names :
[OMP, partial; OMP; OMP]
products gene name :
[OMP]
purity :
>90% in a SDS-PAGE
form :
Dry powder (lyophilized)
storage stability :
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
tested application :
ELISA
app notes :
ELISA: 1:1,200, which-corresponds to 1 f.g/ ml of protein concentration in plates for IgG detection.
image1 heading :
Quality Control
other info1 :
Source: Escherichia coli. Recombinant Antigen: outer membrane channel protein TolC (OMP50) of Salmonella typhi (Parkhill et al., 2001). Molecular Weight: determined by SDS-PAGE, the protein band is between molecular markers of 35,000- 25,000 Da, while relative molecular mass calculated from amino acid sequence is 32,080.30 Da . his-OMP: recombinant antigen with a his-tag in its N-terminus. Total Quantity Per Aliquot: 0.5 mg
other info2 :
Observation: proteins should be maintained frozen at high concentrations. The dilution to be performed for EUSA assays should be made with a small quantity of protein, the same day of the experiment. Once reconstituted and in order to defrost the protein, maintain the aliquot at 2SOC without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface. Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
products categories :
Bacteria
products description :
some of the antigeniC regions of Salmonella typhi antigen OMP has been prepared as a recombinant antigen fused to a his-tag in its N-terminus.
ncbi acc num :
JAO54277.1
ncbi mol weight :
15,896 Da