product summary
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company name :
MyBioSource
product type :
protein
product name :
lipl32 recombinant
catalog :
MBS505042
quantity :
0.1 mg
price :
360 USD
more info or order :
product information
catalog number :
MBS505042
products type :
Recombinant Protein
products full name :
lipl32 recombinant
products short name :
lipl32
products name syn :
RECOMBINANT ANTIGEN LipL32 from Leptospira interrogans; LipL32
products gene name :
lipl32
host :
E Coli
purity :
0.95
form :
Liquid protein solution
concentration :
1.089 mg/ml
storage stability :
Protein is shipped with dry ice. Upon arrival,it should be aliquoted in order to avoid repeated freezingand thawing cycles and stored at -20 degree C to -80 degree C.
tested application :
ELISA (EIA), Western Blot (WB)
app notes :
Recommended concentration in ELISA plates: 0.78 ug/ml in plates for IgM detection. Suggested Titer by ELISA: 1:1400, which corresponds to 0.78 g/ml in plates for IgM detection.
other info1 :
Recombinant Antigen: Leptospira interrogans lipL32 (Haake et al., 2000). Organism: Leptospira interrogans. Tag: HIS, no leader peptide. Molecular Weight Information: Determined by SDS-PAGE, theprotein band is at the molecular marker of 35,000 Da, whilerelative molecular mass calculated from amino acidsequence is 34,961.4 Da. Blast Analysis: NP712818.1. YP_001316. ACZ73827.1. CAE45127.1. ADC80907.1. ADC80908. AAT45368
other info2 :
Observation Notes: Proteins should be maintained frozenat high concentrations. The dilution to be performed forELISA assays should be made with a small quantity ofprotein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking toavoid aggregation. Prior making test dilutions and afterdefrost the protein, is recommended to remove possibleprotein aggregates by centrifuging the stock solution,avoiding alterations in the immobilization of the biomoleculeto the solid surface. Buffer: 20 mM phosphate buffer pH 8 and 1 M NaCl. Batch Composition: Recombinant antigen with a his-tag in its N-terminus. Storage Buffer: 20 mM phosphate buffer pH 8 and 1 M NaCl. his-lipL32: recombinant antigen with a his-tag in its N-terminus. Total volume per aliquot (5% overfill): 0.481ml. Total Quantity Per Aliquot: 0.5mg
products categories :
BACTERIA
products description :
The recombinant antigen LipL32 from Leptospira interrogans has been prepared as the mature antigen fused to a his-tag. It is produced from the complete ORF of the lipL32 gene which codifies the mayor outer membrane protein of the spirochete Leptospira interrogans without the leader peptide.
products references :
Haake, D., G. Chao, R. L. Zuerner, J. K. Barnett, D.Barnett, M. Mazel, J. Matsunaga, P. N. Levett, and C.A. Bolin. The leptospiral major outer membrane proteinLipL32 is a lipoprotein expressed during mammalianinfection. 2000, Infect Immun., 68(4):2276-2285. Gill SC, von Hippel PH. Calculation of protein extinctioncoefficients from amino acid sequence data. AnalBiochem. 1989 Nov 1;182(2):319-26.
ncbi acc num :
NP712818.1, YP_001316, ACZ73827.1, CAE45127.1, ADC80907.1, ADC80908.1, AAT45368
size1 :
0.1 mg
price1 :
360 USD
size2 :
0.5 mg
price2 :
600
size3 :
1 mg
price3 :
850
size4 :
5 mg
price4 :
3285
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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