protein

E protein (DIII) recombinant

MBS505031

1 mg

850 USD

Recombinant Protein

E protein (DIII) recombinant

[E protein (DIII)]

[RECOMBINANT ANTIGEN E from West Nile Virus; E protein (DIII)]

[polyprotein]

Escherichia Coli

3433

Dry powder (lyophilized)

Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.

ELISA (EIA), Western Blot (WB)

Suggested Titer by ELISA: 1:1855, which corresponds to 0.95 ug/ml in plates for IgG, and IgM detection.

Testing Data

Testing Data #2

Testing Data #3

Recombinant Antigen: West Nile virus antigen E (Beasley et al., 2004). Organism: WNV. Molecular Weight Information: Determined by SDS-PAGE, the protein band is between molecular markers of 25,000-18,400 Da, while relative molecular mass calculated from amino acid sequence is 19582,7 Da.

Observation Notes: Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25 degree C without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein, is recommended to remove possible protein aggregates by centrifuging the stock solution, avoiding alterations in the immobilization of the biomolecule to the solid surface. This protein is highly prone to micro-aggregation in absence of 6 M urea, therefore we strongly recommend using fresh protein dilutions in ELISA assay for the protein coating in the microtiter plates. Avoid using storageprotein solutions at 4ºC in absence of 6 M urea. As the pI of the protein is 7, we recommend using carbonate buffer for ELISA plates coating. Batch Composition: his-E: Recombinant antigen with a his-tag in its N-terminus. Note: It is ok with the absence of precipitation after a freezing and thawing cycle. Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.

VIRUSES

the structural domain III of the WNV envelope glycoprotein has been prepared as a recombinant antigen fused to a his-tag at the N-terminal. It is produced from the envelope protein of the West Nile virus.

Beasley et al., 2004. Use of a recombinant anvelope protein subunit antigen for specific serological diagnosis of West Nile virus infection. The Journal of Clinical Microbiology, 42:2759-2765. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.

377814031

AFB76634.1

1 mg

850 USD

5 mg

3285