product summary
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company name :
MyBioSource
product type :
protein
product name :
BZLF1 recombinant
catalog :
MBS505029
quantity :
1 mg
price :
915 USD
more info or order :
MyBioSource product webpage
image
image 1 :
MyBioSource MBS505029 image 1
SDS-page analysis (12.5%) of 2 uL of recombinant zebra. Purity is >98% as determined by gel electrophoresis.
image 2 :
MyBioSource MBS505029 image 2
In this plot, the optical density at 450/620nm for positive (blue) and negative (gray) gG sera are compared for each concentration of the recombinant antigen. An appropriate statistical test of significance for the comparison of means between both groups, the Welch's test, is employed. Eligible concentrations for the use of the antigen should present statistically significant differences between positive and negative sera. This happens when the intervals at the top do not overlap and, equivalently, when the p-value at the bottom is below 0.05. In the present figure, all p-values are below 0.05 and thus the intervals do not overlap. Therefre, any of the showed concentrations can be used to distinguish between positive and negative sera.
product information
catalog number :
MBS505029
products type :
Recombinant Protein
products full name :
BZLF1 recombinant
products short name :
[BZLF1]
products name syn :
[Zebra (EA)]
other names :
[BZLF1]
products gene name :
[BZLF1]
host :
E Coli
sequence length :
245
purity :
12.5%
form :
Dry powder (lyophilized)
storage stability :
Protein is shipped with dry ice. Upon arrival, it should be aliquoted in order to avoid repeated freezing and thawing cycles and stored at -20ºC to -80ºC.
app notes :
ELISA and Western blot assays. Where his product has notbeen tested for use ina particular technique,this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates. . Suggested titer by ELISA 1:2000, which corresponds to 0.93 ug/mlof protein concentration in plates for IgG detection.
image1 heading :
SDS PAGE
image2 heading :
ELISA(E)
other info1 :
MW Note: Determined by SDS-PAGE, the protein band is at the molecular marker is between molecular markers of 45,000 Da and 35,000 kDa , while relative molecular mass calculated from amino acid sequence is 35,081.1 KDa. Composition: Component: his-zebra. Composition:Recombinant antigen with a his-tag in its N-terminus. Buffer: 20mM phosphate buffer pH 8 ,1 M NaCI, and 4 M urea.
other info2 :
Quality Control: Protein concentration determined espectrophometrically. D0 2802 =1.22. 0.1% (=1 g/l)=0.653. Concentration= 1.868 mg/mL. The measurement of the protein concentration has been perfomed with theoretical extinction coefficient of the recomninant protein obtained from Gill and vonHippel, 1989. Total quantity per aliquot: 0.5 mg. Total volume per aliquot: 0.281 mL. Observations: Proteins should be maintained frozen at high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrost the protein, maintain the aliquot at 25ºC without shaking to avoid aggregation. Prior making test dilutions and after defrost the protein,is recomended to remove possible protein aggregates by centrifuging the stock solution, voiding alterations in the immobilization of the biomolecule to the solid surface. Reconstitution: With approximately 0.47 ml of sterile double-distilled water for a final concentration of approximately 1.868mg/ml. The protein will be at pH8.
products categories :
VIRUSES
products description :
Epstein-Barr virus zebra (Yamauchiet al., 1998). The EBVgene BZLF1 has been expressedas a recombinant antigen zebra. It is produced from the complete ORF of the bZIP transcription factor.
ncbi gi num :
808657
ncbi acc num :
AAA66529.1
uniprot summary :
Plays a key role in the switch from latent infection to lytic cycle producing new virions. Acts as a transcription factor, inducing early lytic cycle genes, and as a origin binding protein for genome replication. BZLF1 activates the promoter of another EBV gene (BSLF2+BMLF1).
size1 :
1 mg
price1 :
915 USD
size2 :
2 mg
price2 :
1635
size3 :
5 mg
price3 :
3600
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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