catalog number :
MBS505006
products type :
Recombinant Protein
products full name :
KMP-11 recombinant
products short name :
[KMP-11]
products name syn :
[RECOMBINANT ANTIGEN KMP11 from Leishmania donovani; kinetoplastid membrane protein-11]
other names :
[kinetoplastid membrane protein-11; kinetoplastid membrane protein-11]
products gene name :
[KMP-11]
other gene names :
[KMP11-1]
form :
Dry powder (lyophilized)
concentration :
1.59 mg/ml
storage stability :
Lyophilized protein is shipped with on ice packs. Upon arrival, it should be stored at 20 degree C to -80 degree C. Aliquot after reconstitution in order to avoid repeated freezing and thawing cycles and store at 20 degree C to -80 degree C.
tested application :
ELISA (EIA), Western Blot (WB), Lateral Flow (LF)
app notes :
Suggested Titer by ELISA: approx 1/2,800 which corresponds to 0.56 ug/ml of protein concentration in plates for IgG detection.
image1 heading :
Testing Data
image2 heading :
Testing Data #2
image3 heading :
Testing Data
other info1 :
Recombinant Antigen: Lesihmania donovani KMP11 (Passos et al., 2005). Batch Composition: Components: his-KMP11. Composition: Recombinant antigen with a his-tag in its N-terminus. Molecular Weight Information: Determined by SDS-PAGE, theprotein band is between molecular markers of 25,000 and18,400 Da, while relative molecular mass calculated fromamino acid sequence is 19,460.0 Da
other info2 :
Observation Notes: Proteins should be maintained frozenat high concentrations. The dilution to be performed for ELISA assays should be made with a small quantity of protein, the same day of the experiment. In order to defrostthe protein, maintain the aliquot at 25°C without shaking toavoid aggregation. Prior making test dilutions and afterdefrost the protein, is recommended to remove possibleprotein aggregates by centrifuging the stock solution,avoiding alterations in the immobilization of the biomoleculeto the solid surface. Reconstitution: For lyophilized proteins: with 0.04 ml of sterile double-distilled water (lot specific) for a final concentration of 1.59 mg/ml (lot specific). The protein will be at pH 8.
products categories :
PARASITES
products description :
the Lesihmania donovani antigen KMP-11-biotinylated has been prepared as a recombinant antigenfused to a his-tag and biotinylated "in vivo". It is producedfrom the complete ORF of the KMP-11 gene which codifiesthe kinetoplast membrane protein 11.
products references :
Passos, S., L. P. Carvalho, G. Orge, S. M. Jeronimo, G.Bezerra, M. Soto, C. Alonso, and E. M. Carvalho.Recombinant Leishmania antigens for serodiagnosis ofvisceral leishmaniasis. 2005, Clinical and Diagnostic Lab.Immunology, 12:1164-1167. Bradford, MM. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing theprinciple of protein dye binding. Anal Biochem. 1976,131:499-503.
ncbi acc num :
XP_001469032.1
ncbi gb acc num :
XM_001468995.1