Anti-Phospho-Ser295 Beclin-1 | MyBioSource MBS502207 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser295 Beclin-1

catalog :

MBS502207

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502207

products type :

Antibody

products full name :

Anti-Phospho-Ser295 Beclin-1

products short name :

Beclin1 (Ser295)

other names :

beclin-1; Beclin-1; beclin-1; ATG6 autophagy related 6 homolog; coiled-coil myosin-like BCL2-interacting protein; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1, autophagy related; Coiled-coil myosin-like BCL2-interacting protein; Protein GT197

products gene name :

BECN1

other gene names :

BECN1; BECN1; ATG6; VPS30; beclin1; GT197

uniprot entry name :

BECN1_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human

sequence length :

450

specificity :

Specific for the ~60k beclin1 protein phosphorylated at Ser295. Immunolabeling of the beclin-1 band is eliminated in the beclin-1 alanine substitution mutant.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol.

storage stability :

For long term storage -80 degree C is recommended, but shorter term storage at -20 degree C is al acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at - 20 degree C.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:250

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser295 of human beclin-1. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser295 conjugated to KLH. Immunogen Species: Human

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, mouse, non-human primate, sheep, Xenopus, zebrafish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with recombinant HeLa and 293T cells. It is anticipated that the antibody will react with bovinecanine, chicken, mouse, non-human primate, sheep, Xenopus, and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Akt-mediated phosphorylation of beclin-1 at Ser295 has been shown to decrease autophagy and promote oncogenesis (Wang et al., 2012).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Liang, XH, Jackson S, Seaman M, Brown K, Kempkes B, Hibshoosh H, Levine B. (1999) Induction of autophagy and inhibition of tumorigenesis by beclin-1. Nature 402(6762):672-676. Richard C. Wang, Yongjie Wei, Zhenyi An, Zhongju Zou, Guanghua Xiao, Govind Bhagat, Michael White, Julia Reichelt, and Beth Levine (2012) Akt-Mediated Regulation of Autophagy and Tumorigenesis Through Beclin 1 Phosphorylation. Science 338:956-959.

ncbi gi num :

4502395

ncbi acc num :

NP_003757.1

ncbi gb acc num :

NM_003766.3

uniprot acc num :

Q14457

ncbi mol weight :

ncbi pathways :

Regulation Of Autophagy Pathway (83058); Regulation Of Autophagy Pathway (468); Senescence And Autophagy Pathway (198780)

ncbi summary :

Beclin-1 participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration (Zhong et al., 2009 [PubMed 19270693]).[supplied by OMIM, Jul 2010]

uniprot summary :

Function: Plays a central role in autophagy. Required for the abcission step in cytokinesis. May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. Ref.13. Subunit structure: Interacts with GOPC and GRID2. Interacts with AMBRA1. Forms a complex with PIK3C3 and PIK3R4 and either UVRAG and KIAA0226/Rubicon, or with ATG14. UVRAG and ATG14 form mutually exclusive complexes through direct competition for BECN1-binding. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy . By similarity. Interacts with BCL2 and BCL2L1. Interacts with VMP1. Interacts with PIK3CB . By similarity. Interacts with DAPK1. Interacts with human cytomegalovirus/HHV-5 protein TRS1. Ref.1 Ref.6 Ref.8 Ref.9 Ref.10 Ref.12 Ref.14 Ref.16. Subcellular location: Golgi apparatus trans-Golgi network membrane; Peripheral membrane protein. Note: Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells . By similarity. Ref.9. Tissue specificity: Ubiquitous. Post-translational modification: Phosphorylation at Thr-119 by DAPK1 reduces its interaction with BCL2 and BCL2L1 and promotes induction of autophagy.polyubiquitinated by NEDD4, both with 'Lys11'- and 'Lys63'-linkages. 'Lys'-11-linked poyubiquitination leads to degradation and is enhanced when the stabilizing interaction partner VPS34 is depleted. Deubiquitinated by USP10 and USP13, leading to stabilize the PIK3C3/VPS34-containing complexes. Sequence similarities: Belongs to the beclin family.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage