Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein) | MyBioSource MBS502189 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein)

catalog :

MBS502189

quantity :

0.1 mL

price :

360 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot, immunohistochemistry, dot blot

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS502189

products type :

Antibody

products full name :

Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein)

products short name :

[FMRP (Ser499)]

other names :

[fragile X mental retardation protein 1 homolog; Fragile X mental retardation protein 1 homolog; fragile X mental retardation protein 1 homolog; protein FMR-1; fragile X mental retardation-1 protein; fragile X mental retardation syndrome 1 homolog; fragile X mental retardation 1]

products gene name :

[FMR1]

other gene names :

[Fmr1; Fmr1; FMRP; FMRP]

uniprot entry name :

FMR1_RAT

clonality :

Polyclonal

host :

Rabbit

specificity :

Specific for ~71k FMRP protein phosphorylated at Ser 499 . Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase.

purity :

Affinity Purified

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

storage stability :

For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

tested application :

Western Blot (WB) and Dot blots (DB). Immunohistochemistry (IHC) has not yet been determined.

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000. DB: 1:1000

image1 heading :

Testing Data

products description :

Affinity purified rabbit polyclonal antibody. Fragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome, autism, as well as other cognitive deficits (Brown et al.,1998, Goodlin-Jones et al., 2004). Phosphorylation of the highly conserved Ser499 has been shown to trigger hierarchical phosphorylation of nearby serines and may play a role in suppressing target mRNA translation (Ceman et al., 2003, Narayanan et al. 2008).

products references :

Bernard PB, Castano AM, O'Leary H, Simpson K, Browning MD, Benke TA. (2013) Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiol Dis. Nov; 59:1-17. Brown V, Small K, Lakkis L, Feng Y, Gunter C, Wilkinson KD, Warren ST (1998) Purified recombinant Fmrp exhibits selective RNA binding as an intrinsic property of the fragile X mental retardation protein. J Biol Chem 273:15521-15527. Ceman S, O'Donnell WT, Reed M, Patton S, Pohl J, Warren ST. (2003) Phosphorylation influences the translation state of FMRP-associated polyribosomes. Hum Mol Genet. Dec 15;12(24):3295-305. Goodlin-Jones BL, Tassone F, Gane LW, Hagerman RJ. (2004) Autistic spectrum disorder and the fragile X premutation. J Dev Behav Pediatr. Dec;25(6):392-8. Narayanan U, Nalavadi V, Nakamoto M, Thomas G, Ceman S, Bassell GJ, Warren ST. (2008) S6K1 phosphorylates and regulates fragile X mental retardation protein (FMRP) with the neuronal protein synthesis-dependent mammalian target of rapamycin (mTOR) signaling cascade. J Biol Chem 283:18478-18482

ncbi gi num :

30794228

ncbi acc num :

NP_434691.1

ncbi gb acc num :

NM_052804.1

uniprot acc num :

Q80WE1

ncbi pathways :

RNA Transport Pathway (177822); RNA Transport Pathway (175229)

ncbi summary :

may play a role in mRNA trafficking and localization [RGD, Feb 2006]

uniprot summary :

Function: Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. Involved in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C) . By similarity. Subunit structure: Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain . By similarity. Subcellular location: Cytoplasm . By similarity. Nucleus nucleolus . By similarity. Domain: The tandem Tudor domains preferentially recognize trimethylated histone peptides . By similarity. Post-translational modification: Phosphorylated on several serine residues . By similarity. Miscellaneous: RNA-binding activity is inhibited by RANBP9 . By similarity. Sequence similarities: Belongs to the FMR1 family.Contains 2 Agenet-like domains.Contains 2 KH domains. Sequence caution: The sequence AAB07073.1 differs from that shown. Reason: N-ter sequencing errors.

size1 :

0.1 mL

price1 :

360 USD

more info or order :

MyBioSource product webpage