Anti-Olig2 | MyBioSource MBS502172 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Olig2

catalog :

MBS502172

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502172

products type :

Antibody

products full name :

Anti-Olig2

products short name :

Olig2

other names :

oligodendrocyte transcription factor 2; Oligodendrocyte transcription factor 2; oligodendrocyte transcription factor 2; oligodendrocyte transcription factor 2

products gene name :

OLIG2

other gene names :

Olig2; Olig2; RK17; Olg-2; Bhlhb1; Oligo2; bHLHe19; AI604895; Oligo2

uniprot entry name :

OLIG2_MOUSE

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, mouse, rat

sequence length :

323

specificity :

Specific for the ~32 kDa Olig2 protein in Western blots of human, mouse, and rat tissue. The antibody also works well for immunohistochemistry, immunocytochemistry, and immunoprecipitation.

purity :

Protein A purified (Protein A purified rabbit serum.)

form :

Liquid in 100 mM Glycine, 200 mM Tris, pH 8.0. Contains no preservative.

storage stability :

Store at -20 degree C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:3,000. IHC: 1:1,000. ICC: 1:1,000. IP: 1:500

other info1 :

Antigen: Recombinant mouse Olig2. Immunogen Information: Recombinant mouse Olig2. Immunogen Species: Mouse

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in human, mouse and rat. Other species have not yet been tested. Biological Significance: Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).

products description :

Rabbit polyclonal antibody

products references :

Arnett, H.A., et al. (2004) bHLH transcription factor Olig1 is required to repair demyelinated lesions in the CNS. Science 306(5704):2111-5. Ligon KL, Huillard E, Mehta S, Kesari S, Liu H, Alberta JA, Bachoo RM, Kane M, Louis DN, Depinho RA, Anderson DJ, Stiles CD, Rowitch DH (2007) Olig2 regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma. Neuron 53(4):503-17.

ncbi gi num :

59858557

ncbi acc num :

NP_058663.2

ncbi gb acc num :

NM_016967.2

uniprot acc num :

Q9EQW6

ncbi mol weight :

ncbi pathways :

XPodNet - Protein-protein Interactions In The Podocyte Expanded By STRING Pathway (755427)

uniprot summary :

Function: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development. Ref.5 Ref.6. Subunit structure: Interacts with NKX2-2. Ref.9. Subcellular location: Nucleus. Cytoplasm. Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2. Ref.7 Ref.9. Tissue specificity: Expressed specifically in the brain. Ref.1. Developmental stage: Expressed in the ventral spinal cord as early as 9.5 dpc. Expression becomes progressively restricted to a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. In the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5 dpc onward. Ref.1 Ref.2. Induction: By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain. Ref.4 Ref.8. Domain: The bHLH is essential for interaction with NKX2-2. Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage