antibody

Anti-Phospho-Ser137 DARPP-32

MBS502169

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-Phospho-Ser137 DARPP-32

DARPP-32 (Ser137)

protein phosphatase 1 regulatory subunit 1B; Protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1 regulatory subunit 1B; neuronal phosphoprotein DARPP-32; dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; protein phosphatase 1, regulatory (inhibitor) subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

PPP1R1B

Ppp1r1b; Ppp1r1b; Darpp32; Darpp-32

PPR1B_RAT

Polyclonal

Rabbit

Rat

205

Specific for the ~32k DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen but not by the dephospho-peptide

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -80 degree C is recommended, but shorter term storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 yr at -20 degree C.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser137 of rat DARPP-32. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser137 conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse and non-human primates. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac (Svenningsson et al., 2002).

Affinity purified rabbit polyclonal antibody

Bibb JA, Snyder GL, Nishi A, Yan Z, Meijer L, Fienberg AA, Tsai LH, Kwon YT, Girault JA, Czernik AJ, Huganir RL, Hemmings HC, Jr., Nairn AC, Greengard P (1999) Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons. Nature (London) 402:669-671. Fienberg, A.A., Hiroi, N., Mermelstein, P.G., Song, W., Snyder, G.L., Nishi, A., Cheramy, A. O'Callaghan, J.P., Miller, D.B., Cole, D.G., Corbett, R., Haile, C.N., Cooper, D,C., Onn, S.P., Grace, A.A., Ouimet, C.C., White, F.G., Hyman, S.E., Surmeier, D.G., Girault, J., Nestler, E.J. and Greengard, P. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842. Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G (2002) Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418:774-778. Maldve RE, Zhang TA, Ferrani-Kile K, Schreiber SS, Lippmann MJ, Snyder GL, Feinberg AA, Leslie SW, Gonzales RA, Morrisett RA (2002) DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nature Neurosci 5:641-648. Svenningsson P, Tzavara ET, Witkin JM, Fienberg AA, Nomikos GG, Greengard P (2002) Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac). Proc Natl Acad Sci USA 99:3182-3187.

61889054

NP_612530.1

NM_138521.1

Q6J4I0

32

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Cocaine Addiction Pathway (546271); Cocaine Addiction Pathway (546273); DARPP-32 Events Pathway (936155); Dopaminergic Synapse Pathway (469193); Dopaminergic Synapse Pathway (469185); Opioid Signalling Pathway (936147)

potent inhibitor of protein phosphatase 1 (PP1); may play a role in maintaining the levels of excitability of NMDA receptors [RGD, Feb 2006]

Function: Inhibitor of protein-phosphatase 1 . By similarity. Subcellular location: Cytoplasm . By similarity. Post-translational modification: Phosphorylation of Thr-34 is required for activity . By similarity.Dopamine- and cyclic AMP-regulated neuronal phosphoprotein . By similarity. Sequence similarities: Belongs to the protein phosphatase inhibitor 1 family.

0.1 mL

345 USD