Anti-Periostin, C-terminal | MyBioSource MBS502166 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Periostin, C-terminal

catalog :

MBS502166

quantity :

0.1 ml

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, chicken

application :

western blot, immunohistochemistry

product information

catalog number :

MBS502166

products type :

Antibody

products full name :

Anti-Periostin, C-terminal

products short name :

Periostin C-terminal

other names :

periostin isoform 2; Periostin; periostin; periostin-like factor; osteoblast-specific factor 2; osteoblast specific factor 2 (fasciclin I-like); periostin, osteoblast specific factor; Osteoblast-specific factor 2

products gene name :

POSTN

other gene names :

Postn; Postn; PN; PLF; Osf2; peri; OSF-2; AI747096; A630052E07Rik; Osf2; PN; OSF-2

uniprot entry name :

POSTN_MOUSE

clonality :

Polyclonal

host :

Rabbit

reactivity :

Chicken, human, mouse

sequence length :

810

specificity :

Specific for the ~93 kDa periostin doublet in mouse lung extract. Consistent with the fact that this antibody is made against an alternatively spliced region of periostin, this antibody recognizes only the largest two of the three forms of periostin recognized on Western blots by the Pan periostin antibody and also shows a distinctive staining pattern by immunohistochemistry (data not shown). The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90 degree C in 10 mM sodium citrate (pH 6.0)/ 0.1 % Tween-20).

purity :

Affinity Purified

form :

Liquid in PBS. Contains no preservative.

storage stability :

Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1,000. IHC: 1:100

other info1 :

Antigen: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons. Immunogen Information: Bacterial fusion protein equivalent to a 188-amino acid polypeptide from the C-terminal region of mouse periostin which is comprised of six small alternatively-spliced exons. Immunogen Species: Mouse

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Most mammalian and avian. Species Reactivity Note: The antibody has been directly tested for reactivity in chicken, human, mouse and rat. The antibody is expected to work on all mammalian and avian species. Biological Significance: Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has since been demonstrated to be important in heart valve development and myocardial infarction because it promotes collagen fibrogenesis, inhibits differentiation of progenitor cells into cardiomyocytes and is essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008).

products description :

Rabbit polyclonal antibody

products references :

Kruzynska-Frejtag A, Wang J, Rogers R, Krug E, Hoffman S, Markwald RR, Conway SJ. (2004) Periostin is expressed within the developing teeth at the sites of epithelial-mesenchymal interaction. Develop Dynamics, 229:857-868. Norris RA, Borq TK, Butcher JT, Baudino TA, Banerjee I, Markwald RR (2008) Neonatal and adult cardiovascular pathophysiological remodeling and repair: developmental role of periostin. Ann NY Acad Sci. 1123:30-40. Norris RA, Damon B, Mironov V, Kasyanov V, Ramamurthi A, Moreno-Rodriguez R, Trusk T, Potts JD, Goodwin RL, Davis J, Hoffman S, Wen X, Sugi Y, Kern CB, Mjaatvedt CH, Turner DK, Oka T, Conway SJ, Molkentin JD, Forgacs G, Markwald RR. (2007) Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues. J Cell Biochem. 101(3):695-711.

ncbi gi num :

311771596

ncbi acc num :

NP_001185694.1

ncbi gb acc num :

NM_001198765.1

uniprot acc num :

Q62009

ncbi mol weight :

uniprot summary :

Function: Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX. Ref.4 Ref.8. Subunit structure: Interacts with BMP1 and fibronectin. Ref.8. Subcellular location: Golgi apparatus. Secreted extracellular space extracellular matrix. Note: Colocalizes with BMP1 in the Golgi. Ref.4 Ref.8. Tissue specificity: Preferentially expressed in periosteum and periodontal ligament. Also expressed in the developing and adult heart. Ref.4 Ref.6. Developmental stage: In the heart, expressed from embryonic day 10.5. Continues to be strongly expressed throughout cardiac development and into adulthood. Ref.6. Induction: By TGF-beta. Ref.4. Post-translational modification: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium. Disruption phenotype: Reduced amount of collagen cross-linking in femur and periosteum. Ref.8. Sequence similarities: Contains 1 EMI domain.Contains 4 FAS1 domains.

size :

0.1 ml

price :

270 USD