antibody

Anti-Phospho-Tyr1325 NMDA NR2A-Subunit

MBS502160

0.1 ml

270 USD

polyclonal

rabbit

nonconjugated

mouse, rat

Antibody

Anti-Phospho-Tyr1325 NMDA NR2A-Subunit

NMDA NR2A Subunit (Tyr1325)

glutamate receptor ionotropic, NMDA 2A; Glutamate receptor ionotropic, NMDA 2A; glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1; glutamate receptor, ionotropic, N-methyl D-aspartate 2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A

GRIN2A

Grin2a; Grin2a; NR2A; GluN2A; NMDAR2A; GluN2A; NMDAR2A; NR2A

NMDE1_RAT

Polyclonal

Rabbit

Mouse, rat

1464

Specific for the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely blocked by blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of rat NMDA NR2A. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1325 conjugated to KLH. Immunogen Species: Rat

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse and rat tissues. It is anticipated that the antibody will react with bovine and canine based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).

Affinity purified rabbit polyclonal antibody

Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003)Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Ishii T, Moriyoshi K, Sugihara H, Sakurada K, Kadotani H, Yokoi M, Akazawa C, Shigemoto R, Mizuno N, Masu M, Nakanishi S (1993) Molecular characterization of the family of the N-methyl- D-aspartate receptor subunits. J Biol Chem 268:2836-2843. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. Snell LD, Nunley KR, Lickteig RL, Browning MD, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Mol Brain Res 40:71-78. Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annu Rev Pharmacol Toxicol 43:335-358. King JE, Eugenin EA, Hazleton JE, Morgello S, Berman JW (2010) Mechanisms of HIV-tat-induced phosphorylation of N-methyl-D-aspartate receptor subunit 2A in human primary neurons: implications for neuroAIDS pathogenesis. Am J Pathol. 176(6):2819-30. Taniguchi S, Nakazawa T, Tanimura A, Kiyama Y, Tezuka T, Watabe AM, Katayama N, Yokoyama K, Inoue T, Izumi-Nakaseko H, Kakuta S, Sudo K, Iwakura Y, Umemori H, Inoue T, Murphy NP, Hashimoto K, Kano M, Manabe T, Yamamoto T. (2009) Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour. EMBO J. 28(23):3717-29.

140971205

NP_036705.3

NM_012573.3

Q00959

180

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway 936445!!Alcoholism Pathway 585585!!Alcoholism Pathway 587116!!Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Amphetamine Addiction Pathway 547642!!Amphetamine Addiction Pathway 550546!!Amyotrophic Lateral Sclerosis (ALS) Pathway 83491!!Amyotrophic Lateral Sclerosis (ALS) Pathway 511!!CREB Phosphorylation Through The Activation Of CaMKII Pathway 936452

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with AIP1. Found in a complex with GRIN1 and GRIN3B. Interacts with HIP1 and NETO1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes . By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with LRFN2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein Ref.8. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. [View classification]

0.1 ml

270 USD