antibody

Anti-DsbA-L

MBS502156

0.1 ml

235 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Anti-DsbA-L

DsbA-L (Disulfide-bond-A oxidoreductase-like protein)

Polyclonal

Rabbit

Mouse, human

Specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and mouse white and brown adipose tissue lysate samples. It is suggested that you add 1% BSA to the antibody dilution buffer.

Serum

Liquid in 40% glycerol.

Store at -20 degree C in undiluted aliquots; stable for at least 1 year after date of receipt. Avoid freeze/thaw cycles.

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:2000. IHC: 1:200

Antigen: Full length recombinant mouse DsbA-L protein. Immunogen Information: Full length recombinant mouse DsbA-L protein. Immunogen Species: Mouse

Species Reactivity Note: The antibody has been directly tested for reactivity in mouse and human. Other species have not been tested. Biological Significance: Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution (Gao F et al., 2009). Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis (Liu et al., 2008).

Rabbit polyclonal antibody

Polymorphism of DsbA-L gene associates with insulin secretion and body fat distribution in Chinese population. (2009) Gao F, Fang Q, Zhang R, Lu J, Lu H, Wang C, Ma X, Xu J, Jia W, Xiang K. Endocr J. Jun;56(3):487-94. Liu et al, (2008) A disulfide-bond A oxidoreductase-like protein (DsbA-L) regulates adiponectin multimerization. Proc Natl Acad Sci USA, 105, 18302-18307. Wang A, Liu M, Liu X, Dong LQ, Glickman RD, Slaga TJ, Zhou Z, Liu F (2011) Up-regulation of Adiponectin by Resveratrol: THE ESSENTIAL ROLES OF THE Akt/FOXO1 AND AMPACTIVATED PROTEIN KINASE SIGNALING PATHWAYS AND DsbA-L. J Biol Chem. Jan 7;286(1):60-6.

25

0.1 ml

235 USD