antibody

Anti-Choline Acetyltransferase

MBS502147

0.1 mL

345 USD

polyclonal

goat

nonconjugated

human, mouse, chicken

Antibody

Anti-Choline Acetyltransferase

ChAT (Choline Acetyltransferase)

choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase; choline O-acetyltransferase

CHAT

CHAT; CMS1A; CMS1A2; CHOACTASE

Polyclonal

Goat

Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates

Specific for the ~ 70k choline acetyltransferase protein

Affinity Purified (Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.)

100 ul in PBS + 5 mg/ml BSA + 0.2% NaN3. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:100

Antigen: Native choline acetyltransferase purified from human placenta. Immunogen Information: Native choline acetyltransferase purifed from human placenta. Immunogen Species: Human

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with chicken, guinea pig, human, mouse, non-human primate and rat tissues. Biological Significance: Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).

Affinity purified goat polyclonal antibody

Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459. Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31. Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.

P28329.

70

Acetylcholine Neurotransmitter Release Cycle Pathway (106525); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Neuronal System Pathway (106513)

This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]

CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described. Protein type: Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.6. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: cell soma; mitochondrion; axon; cytoplasm; cytosol; nucleus. Molecular Function: choline O-acetyltransferase activity. Biological Process: synaptic transmission; rhythmic excitation; muscle development; rhythmic behavior; phospholipid metabolic process; neurotransmitter secretion; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process. Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea

0.1 mL

345 USD