Anti-Microtubule Associated Protein 2 (MAP2) | MyBioSource MBS502140 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Microtubule Associated Protein 2 (MAP2)

catalog :

MBS502140

quantity :

0.1 mL

price :

300 USD

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502140

products type :

Antibody

products full name :

Anti-Microtubule Associated Protein 2 (MAP2)

products short name :

Microtubule Associated Protein 2 (MAP2)

other names :

microtubule-associated protein 2 isoform 5; Microtubule-associated protein 2; microtubule-associated protein 2; MAP-2; microtubule-associated protein 2

products gene name :

MAP2

other gene names :

MAP2; MAP2; MAP2A; MAP2B; MAP2C; MAP-2

uniprot entry name :

MTAP2_HUMAN

clonality :

Polyclonal

host :

Chicken

reactivity :

Most Mammals

sequence length :

559

specificity :

Specific for the ~ 280k MAP2 protein in Western Blots of rat brain homogenate.

purity :

Total IgY fraction

form :

Liquid containing 10mM sodium azide

storage stability :

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Quality Control Test:. Western blots performed on each lot. WB: 1:20,000. IF: 1:2,500

other info1 :

Antigen: Bovine MAP2 isolated from brain by the GTP microtubule cycling method. Immunogen Species: bovine. Packaging: 100ul antibody per vial +10 mM NaN3. Adequate amount of material to conduct 10-mini Wester Blots.

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues. Biological Significance: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein tau (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein tau (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006).

products description :

Chicken polyclonal antibody

products references :

Kindler S, Gardner CC (1994) Four repeat MAP2 isoforms in human and rat brain. Brain Res Mol Brain Res. 26(1-2):218-224. Fontaine-Lenore V, Chambraud B, Fellous A, David S, Duchossoy Y, Baulieu EE, Robel P (2006) Microtubule-associated protein 2 (MAP2) is a neurosteroid. Proc Natl Acad Sci USA 103(12):4711-6.

ncbi gi num :

87578394

ncbi acc num :

NP_001034627.1

ncbi gb acc num :

NM_001039538.1

uniprot acc num :

P11137

ncbi mol weight :

280

ncbi pathways :

LKB1 Signaling Events Pathway (137957); MAPK Cascade Pathway (198834); SIDS Susceptibility Pathways (198901)

ncbi summary :

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

uniprot summary :

Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Subcellular location: Cytoplasm cytoskeleton . Probable. Post-translational modification: Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly . By similarity. MAP2A/c is phosphorylated. Isoform MAP2cis phosphorylated by FYN at Tyr-67. Ref.8. Sequence similarities: Contains 3 Tau/MAP repeats.

size1 :

0.1 mL

price1 :

300 USD

more info or order :

MyBioSource product webpage