Anti-Alpha-Internexin (NF66) | MyBioSource MBS502138 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Alpha-Internexin (NF66)

catalog :

MBS502138

quantity :

0.1 ml

price :

235 USD

clonality :

polyclonal

host :

chicken

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502138

products type :

Antibody

products full name :

Anti-Alpha-Internexin (NF66)

products short name :

Alpha Internexin/ NF-66

other names :

alpha-internexin; Alpha-internexin; alpha-internexin; alpha-Inx; internexin, alpha; internexin neuronal intermediate filament protein, alpha

products gene name :

INA

other gene names :

Ina; Ina; Nf66; Inexa; Intlaa; Inexa; Alpha-Inx

uniprot entry name :

AINX_RAT

clonality :

Polyclonal

host :

Chicken

reactivity :

Mouse, human, rat, many other mammals

sequence length :

505

specificity :

Specific for the ~66k alpha Internexin protein. Minor bands at ~150k are probably covalent dimers and bands at ~50k represent alpha-internexin breakdown products.

purity :

Total IgY fraction

form :

Liquid containing 10mM sodium azide.

storage stability :

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

tested application :

Western Blot (WB), Immunofluorescence (IF)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:5,000. IF: 1:500

other info1 :

Antigen: Recombinant rat alpha-internexin expressed in and purified from E. coli. Immunogen Information: Recombinant rat alpha-internexin expressed in and purified from E. coli. Immunogen Species: Rat

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in mouse, human and rat tissues. Biological Significance: Alpha-internexin is a Class IV intermediate filament originally discovered as it co-purifies with other neurofilament subunits (1). Alpha-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development proceeds. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition, recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Cairns et al. used this antibody to show that alpha-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID), a serious human neurodegenerative disorder (3,4). The antibody was also used to confirm the presence of circulating auto-antibodies to alpha-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (5).

products description :

Chicken polyclonal antibody

products references :

Pachter, J and Liem, RKH. Alpha-Internexin, a 66-kD intermediate filament-binding protein from mammalian central nervous tissues. J Cell Biol 101:1316-22 (1985). McGraw et al. Axonally transported peripheral signals regulate alpha-internexin expression in regenerating motoneurons. J Neurosci 22:4955-63 (2002). Cairns NJ et al. alpha-Internexin aggregates are abundant in neuronal intermediate filament inclusion disease (NIFID) but rare in other neurodegenerative diseases. Acta Neuropathol (Berl). May 28 [Epub ahead of print] (2004). Cairns NJ et al. alpha-internexin is present in the pathological inclusions of neuronal intermediate filament inclusion disease. Am J Pathol. 164:2153-61 (2004). Rajasalu T, Teesalu K, Janmey PA, Uibo R. Demonstration of natural autoantibodies against the neurofilament protein alpha-internexin in sera of patients with endocrine autoimmunity and healthy individuals. Immunol Lett. 94:153-60 (2004).

ncbi gi num :

9506811

ncbi acc num :

NP_062001.1

ncbi gb acc num :

NM_019128.4

uniprot acc num :

P23565

ncbi mol weight :

ncbi summary :

may play a role in neurogenesis; may be involved in neuronal regeneration in response to injury [RGD, Feb 2006]

uniprot summary :

Function: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges . By similarity. Developmental stage: Levels of this protein reach a maximum at embryonic day 16 and decline into adulthood. Post-translational modification: O-glycosylated . By similarity. Sequence similarities: Belongs to the intermediate filament family.

size :

0.1 ml

price :

235 USD

more info or order :

MyBioSource product webpage