Anti-Vesicular GABA Amino Acid Transporter (VGAT) | MyBioSource MBS502136 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Vesicular GABA Amino Acid Transporter (VGAT)

catalog :

MBS502136

quantity :

0.1 ml

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502136

products type :

Antibody

products full name :

Anti-Vesicular GABA Amino Acid Transporter (VGAT)

products short name :

Vesicular GABA transporter (VGAT)

other names :

vesicular inhibitory amino acid transporter; Vesicular inhibitory amino acid transporter; vesicular inhibitory amino acid transporter; rGVAT; vesicular GABA transporter; GABA and glycine transporter; solute carrier family 32 member 1; solute carrier family 32 (GABA vesicular transporter), member 1; GABA and glycine transporter; Solute carrier family 32 member 1; Vesicular GABA transporter

products gene name :

SLC32A1

other gene names :

Slc32a1; Slc32a1; Vgat; Viaat; Vgat; Viaat; rGVAT

uniprot entry name :

VIAAT_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

525

specificity :

Specific for the ~53k VGAT protein.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification on a column made with the peptide used as antigen.)

form :

Packaging: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

Store at -20 degree C; stable for at least one year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Peptide corresponding to amino acid residues from the N-terminal region of rat Vesicular GABA Amino Acid Transporter (VGAT). Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine and non-human primate. Species Reactivity Note: The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with bovine, canine and non-human primate tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: The Vesicular GABA Amino Acid Transporter (VGAT) is responsible for transport of the inhibitory neurotransmitter into synaptic vesicles(McIntire et al., 1997). The VGAT protein (also known as the Vesicular Inhibitory Amino Aid Transporter or VIAAT) is expressed in synaptic vesicles of both glycine and GABAergic synapses throughout the CNS (Chaudhry et al., 1998). Expression of the VGAT protein changes during development and also in response to patterns of neuronal activity (De et al., 2005).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Chaudhry FA, Reimer RJ, Bellocchio EE, Danbolt NC, Osen KK, Edwards RH, Storm-Mathisen J (1998) The vesicular GABA transporter, VGAT, localizes to synaptic vesicles in sets of glycinergic as well as GABAergic neurons. J Neurosci 18:9733-9750. De GS, Schafer MK, Defamie N, Chen C, Ricci A, Weihe E, Varoqui H, Erickson JD (2005) Homeostatic scaling of vesicular glutamate and GABA transporter expression in rat neocortical circuits. J Neurosci 25:7121-7133. McIntire SL, Reimer RJ, Schuske K, Edwards RH, Jorgensen EM (1997) Identification and characterization of the vesicular GABA transporter. Nature (Lond) 389:870-876.

ncbi gi num :

13929106

ncbi acc num :

NP_113970.1

ncbi gb acc num :

NM_031782.1

uniprot acc num :

O35458

ncbi mol weight :

ncbi pathways :

GABA Synthesis, Release, Reuptake And Degradation Pathway 936421!!GABAergic Synapse Pathway 377244!!GABAergic Synapse Pathway 377129!!Morphine Addiction Pathway 552694!!Morphine Addiction Pathway 554808!!Neuronal System Pathway 936410!!Neurotransmitter Release Cycle Pathway 936415!!Nicotine Addiction Pathway 583273!!Nicotine Addiction Pathway 584676!!Retrograde Endocannabinoid Signaling Pathway 537882

ncbi summary :

integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles [RGD, Feb 2006]

uniprot summary :

Function: Involved in the uptake of GABA and glycine into the synaptic vesicles. Subcellular location: Cytoplasmic vesicle membrane; Multi-pass membrane protein. Tissue specificity: Brain. Expressed at high levels within the neocortex, hippocampus, cerebellum, striatum, septal nuclei and the reticular nucleus of the thalamus. Also expressed in islets where it is more abundant in the peripheral/mantle region. Sequence similarities: Belongs to the amino acid/polyamine transporter 2 family.

size :

0.1 ml

price :

270 USD

more info or order :

MyBioSource product webpage