Anti-Phospho-Ser31 Tyrosine Hydroxylase | MyBioSource MBS502133 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser31 Tyrosine Hydroxylase

catalog :

MBS502133

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502133

products type :

Antibody

products full name :

Anti-Phospho-Ser31 Tyrosine Hydroxylase

products short name :

Tyrosine Hydroxylase (Ser31)

other names :

tyrosine 3-monooxygenase; Tyrosine 3-monooxygenase; tyrosine 3-monooxygenase; tyrosine 3-hydroxylase; tyrosine hydroxylase; Tyrosine 3-hydroxylase

products gene name :

other gene names :

Th; Th; The; TH

uniprot entry name :

TY3H_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat, mouse

sequence length :

498

specificity :

Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser31.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections; Witkovsky et al., 2000): 1:1000. IF (frozen sections; Witkovsky et al., 2000): 1:1000

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser31 of rat tyrosine hydroxylase (TH). Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Non-human primates. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with non-human primate tissues based on the fact that this species has 100% homology with the amino acid sequence used as antigen. Biological Significance: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.

products description :

Affinity purified rabbit polyclonal antibody

products references :

Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043. Haycock JW, Ahn NG, Cobb MH, Krebs EG (1992) ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci (USA) 89:2365-2369. Haycock JW, Bennett WF, George RJ, Waymire JC (1982) Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257:13699-13703. Jedynak JP, Ali SF, Haycock JW, Hope BT (2002) Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and-40 in tyrosine hydroxylase. J Neurochem 82:382-388. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.

ncbi gi num :

6981652

ncbi acc num :

NP_036872.1

ncbi gb acc num :

NM_012740.3

uniprot acc num :

P04177

ncbi mol weight :

ncbi pathways :

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (935647); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198489); Catecholamine Biosynthesis Pathway (935648); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (434674); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Catecholamine Synthesis Pathway (198533)

ncbi summary :

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

uniprot summary :

Function: Plays an important role in the physiology of adrenergic neurons. Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2. Subunit structure: Homotetramer. Post-translational modification: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation. Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage