Anti-Phospho-Ser58 Tryptophan Hydroxylase 1 | MyBioSource MBS502129 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser58 Tryptophan Hydroxylase 1

catalog :

MBS502129

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502129

products type :

Antibody

products full name :

Anti-Phospho-Ser58 Tryptophan Hydroxylase 1

products short name :

Tryptophan Hydroxylase (Ser58)

other names :

tryptophan 5-hydroxylase 1; Tryptophan 5-hydroxylase 1; tryptophan 5-hydroxylase 1; tryptophan 5-monooxygenase 1; tryptophan hydroxylase 1; Tryptophan 5-monooxygenase 1

products gene name :

TPH1

other gene names :

Tph1; Tph1; Tph; Tph

uniprot entry name :

TPH1_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rabbit

sequence length :

444

specificity :

Specific for the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser58 of tryptophan Hydroxylase 1 (TPH1). Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine chicken, human, mouse non-human primate, rat xenopus zebra fish . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rabbit tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primate, rat, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Tryptophan hydroxylase (TPH) catalyzes the 5-hydroxylation of tryptophan, which is the first step in the biosynthesis of indoleamines (serotonin and melatonin) (Martinez et al., 2001). In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland. Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis. Serotonin functions mainly as a neurotransmitter, whereas melatonin is the principal hormone secreted by the pineal gland. The activity of TPH is enhanced by phosphorylation by cAMP-dependent protein kinase (PKA) and Ca2+/calmodulin kinase II (CaM K II) (Jiang et al., 2000; Johansen et al., 1996) Both PKA and CaM K II phosphorylate Ser58 which lies within the regulatory domain of TPH (Kuhn et al., 1997).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Jiang GC, Yohrling GJ, Schmitt JD, Vrana KE (2000) Identification of substrate orienting and phosphorylation sites within tryptophan hydroxylase using homology-based molecular modeling. J Mol Biol 302:1005-1017. Johansen PA, Jennings I, Cotton RG, Kuhn DM (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J Neurochem 66:817-823. Kuhn, DM, Arthur, Jr, R, States, JC (1997) Phosphorylation and activation of brain tryptophan hydroxylase: identification of serine-58 as a substrate site for protein kinase A. J Neurochem 68:2220-2223. Martinez A, Knappskog PM, Haavik J (2001) Structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.

ncbi gi num :

158186610

ncbi acc num :

NP_001094104.1

ncbi gb acc num :

NM_001100634.2

uniprot acc num :

P09810

ncbi mol weight :

ncbi pathways :

Amine-derived Hormones Pathway (935647); Biogenic Amine Synthesis Pathway (198489); Melatonin Biosynthesis, Tryptophan = Serotonin = Melatonin Pathway (434672); Melatonin Biosynthesis, Tryptophan = Serotonin = Melatonin Pathway (468230); Metabolism Pathway (935443); Metabolism Of Amino Acids And Derivatives Pathway (935631); Serotonergic Synapse Pathway (525343); Serotonin And Melatonin Biosynthesis Pathway (935651); Tryptophan Metabolism Pathway (83356); Tryptophan Metabolism Pathway (198467)

ncbi summary :

catalyzes the conversion of l-tryptophan to 5-hydroxy-l-tryptophan; member of pterin-dependent aromatic amino acid hydroxylase family [RGD, Feb 2006]

uniprot summary :

Catalytic activity: L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Pathway: Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. Subunit structure: Homotetramer. Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.Contains 1 ACT domain.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage