antibody

Anti-Tryptophan Hydroxylase

MBS502127

0.1 ml

270 USD

polyclonal

sheep

nonconjugated

human, rat

western blot, immunohistochemistry

Antibody

Anti-Tryptophan Hydroxylase

Tryptophan Hydroxylase

tryptophan 5-hydroxylase 2; Tryptophan 5-hydroxylase 1; tryptophan 5-hydroxylase 2; Tryptophan 5-monooxygenase 1

TPH1

TPH2; TPH1; TPH

TPH1_RABIT

Polyclonal

Sheep

Human, rat

444

Specific for the ~55k tryptophan hydroxylase protein.

Affinity Purified (Prepared from sheep serum by affinity purification using a column to which the recombinant protein was coupled.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections, Haycock et al., 2002): 1:1000

Antigen: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. Immunogen Information: Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE. Immunogen Species: Rabbit

Reactivity Assumed Based on 100% Sequence Homology: Wide variety mammalian. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human and rat tissue. Based upon the relatively high degree of homology of tryptophan hydroxylase, the antibodies should cross-react with other mammalian species. Does not recognize TPH in rabbit tissues. Biological Significance: Tryptophan hydroxylase (TPH) catalyzes the first step in the biosynthesis of serotonin and melatonin (Martinez et al., 2001). Thus, expression of TPH can be used as an indicator of the localization of serotonin and melatonin in brain. In mammals, serotonin biosynthesis occurs predominantly in neurons which originate in the Raphe nuclei of the brain, and melatonin synthesis takes place within the pineal gland (Haycock et al., 2002). Although TPH catalyzes the same reaction within the Raphe nuclei and the pineal gland, TPH activity is rate-limiting for serotonin but not melatonin biosynthesis (Martinez et al., 2001).

Affinity purified sheep polyclonal antibody

Haycock JW, Kumer SC, Lewis DA, Vrana KE, Stockmeier CA (2002) A monoclonal antibody to tryptophan hydroxylase: applications and identification of the epitope. J Neurosci Methods 114:205-212. Martinez A, Knappskog PM, Haavik J (2001) A structural approach into human tryptophan hydroxylase and its implications for the regulation of serotonin biosynthesis. Curr Med Chem 8:1077-1091.

126723694

NP_001075741.1

NM_001082272.1

P17290

55

Catalytic activity: L-tryptophan + tetrahydrobiopterin + O2 = 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin. Cofactor: Fe2+ ion. Pathway: Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2. Subunit structure: Homotetramer. Ref.3. Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.Contains 1 ACT domain.

0.1 ml

270 USD