antibody

Anti-Phospho-Ser181 TAO2

MBS502125

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

phosphorylated

human, mouse

Antibody

Anti-Phospho-Ser181 TAO2

TAO2 (Ser181)

serine/threonine-protein kinase TAO2 isoform 3; Serine/threonine-protein kinase TAO2; serine/threonine-protein kinase TAO2; PSK-1; hKFC-C; kinase from chicken homolog C; prostate-derived STE20-like kinase 1; prostate derived STE20-like kinase PSK; prostate-derived sterile 20-like kinase 1; thousand and one amino acid protein kinase 2; TAO kinase 2; Kinase from chicken homolog C; hKFC-C; Prostate-derived sterile 20-like kinase 1; PSK-1; PSK1; Prostate-derived STE20-like kinase 1; Thousand and one amino acid protein kinase 2

TAOK2

TAOK2; TAOK2; PSK; PSK1; TAO1; TAO2; MAP3K17; PSK1-BETA; KIAA0881; MAP3K17; PSK; PSK1; hKFC-C; PSK-1; PSK1

TAOK2_HUMAN

Polyclonal

Rabbit

Human, mouse, rat

1122

Specific for the ~120k TAO2 phosphorylated at Ser181 in Western blots. Immunolabeling is completely eliminated by treatment with lambda phosphatase.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser181 of TAO2. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 conjugated to KLH. Immunogen Species: Human

Reactivity Assumed Based on 100% Sequence Homology: Zebra fish xenopus finch canine . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue. It is anticipated that the antibody will react with canine, finch, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

Affinity purified rabbit polyclonal antibody

Chen Z, Raman M, Chen L, Lee SF, Gilman AG, Cobb MH (2003) TAO (thousand-and-one amino acid) protein kinases mediate signaling from carbachol to p38 mitogen-activated protein kinase and ternary complex factors. J Biol Chem 278:22278-22283. Chen Z, Cobb, MH (2001) Regulation of stress-responsive mitogen-activated protein (MAP) kinase pathways by TAO2. J Biol Chem 276:16070-16075. Chen Z, Hutchison M, Cobb MH (1999) Isolation of the protein kinase TAO2 and identification of its mitogen-activated protein kinase/extracellular signal-regulated kinase kinase binding domain. J Biol Chem 274:28803-28807.

354721195

NP_001238972.1

NM_001252043.1

Q9UL54

120

MAPK Signaling Pathway (198779); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); P38 MAPK Signaling Pathway (137943)

This gene encodes a serine/threonine protein kinase that is involved in many different processes, including, cell signaling, microtubule organization and stability, and apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Function: Serine/threonine-protein kinase involved in different processes such as membrane blebbing and apoptotic bodies formation DNA damage response and MAPK14/p38 MAPK stress-activated MAPK cascade. Phosphorylates itself, MBP, activated MAPK8, MAP2K3, MAP2K6 and tubulins. Activates the MAPK14/p38 MAPK signaling pathway through the specific activation and phosphorylation of the upstream MAP2K3 and MAP2K6 kinases. In response to DNA damage, involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. Isoform 1, but not isoform 2, plays a role in apoptotic morphological changes, including cell contraction, membrane blebbing and apoptotic bodies formation. This function, which requires the activation of MAPK8/JNK and nuclear localization of C-terminally truncated isoform 1, may be linked to the mitochondrial CASP9-associated death pathway. Isoform 1 binds to microtubules and affects their organization and stability independently of its kinase activity. Prevents MAP3K7-mediated activation of CHUK, and thus NF-kappa-B activation, but not that of MAPK8/JNK. May play a role in the osmotic stress-MAPK8 pathway. Isoform 2, but not isoform 1, is required for PCDH8 endocytosis. Following homophilic interactions between PCDH8 extracellular domains, isoform 2 phosphorylates and activates MAPK14/p38 MAPK which in turn phosphorylates isoform 2. This process leads to PCDH8 endocytosis and CDH2 cointernalization. Both isoforms are involved in MAPK14 phosphorylation. Ref.1 Ref.2 Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium . By similarity. Enzyme regulation: Selectively inhibited by the enantiopure organoruthenium inhibitor 9E1. Activated following arsenic trioxide (As2O3) treatment. Ref.17 Ref.19. Subunit structure: Interacts with MAP2K3 and MAP2K6 . By similarity. Self-associates. Interacts with tubulins through the C-terminal domain. Interacts with MAP3K7 and interfers with MAP3K7-binding to CHUK and thus prevents NF-kappa-B activation. Isoform 2 interacts with PCDH8; this complex may also include CDH2 . By similarity. Ref.10 Ref.11 Ref.13. Subcellular location: Cytoplasmic vesicle membrane; Multi-pass membrane protein . Potential. Cytoplasm cytoskeleton. Nucleus. Note: Catalytically active full-length phosphorylated isoform 1 localizes to microtubules in the cytoplasm predominantly on microtubule cables positioned around the nucleus. A C-terminally truncated form of isoform 1 is present in the nucleus; isoform 2 and kinase-defective, as well as full-length isoform 1 are excluded from the nucleus. Ref.2 Ref.11 Ref.15Isoform 2: Cell projection dendrite. Note: In dendrites, colocalizes with PCDH8 . By similarity. Ref.2 Ref.11 Ref.15. Tissue specificity: Ubiquitously expressed, with a higher level of expression in testis and brain. Ref.1 Ref.2. Post-translational modification: Isoforms 1 and 2 are autophosphorylated. Ref.14 Ref.15C-terminal cleavage of isoform 1 and subsequent nuclear localization requires CASP9 activity.Autophosphorylated. Phosphorylated by ATM.Isoform 2:Phosphorylated on Ser-1031 by MAPK14. This phosphorylation is required PCDH8 for endocytosis . By similarity. Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 protein kinase domain. Sequence caution: The sequence AAQ89301.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence BAA74904.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

0.1 mL

345 USD