Anti-Synapsin I | MyBioSource MBS502117 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Synapsin I

catalog :

MBS502117

quantity :

0.01 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, cow

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502117

products type :

Antibody

products full name :

Anti-Synapsin I

products short name :

Synapsin I

other names :

synapsin-1; Synapsin-1; synapsin-1; Synapsin I

products gene name :

SYN1

other gene names :

SYN1; SYN1

uniprot entry name :

SYN1_BOVIN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, mouse, rat

sequence length :

706

specificity :

Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the native protein was coupled.)

form :

10 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody sh

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

tested application :

Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:1000. IF: 1:1000. IP: 1 ug per 200 ug lysate

other info1 :

Antigen: Native protein purified from bovine brain. Immunogen Information: Native protein purified from bovine brain. Immunogen Species: bovine

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat, mouse and human tissue. Biological Significance: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380. Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953. Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437. Moore RY, Bernstein M (1989) Synaptogenesis in the rat suprachiasmatic nucleus demonstrated by electron microscopy and synapsin I immunoreactivity. J Neurosci 9:2151-2162. Nayak AS, Moore CI, Browning MD (1996) CaM kinase II phosphorylation of the presynaptic protein synapsin is persistently increased during expression of long-term potentiation. Proc Natl Acad Sci (USA) 93:15451-15456.

ncbi gi num :

27806393

ncbi acc num :

NP_776616.1

ncbi gb acc num :

NM_174191.2

uniprot acc num :

P17599

ncbi mol weight :

uniprot summary :

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level . By similarity. Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP . By similarity. Subcellular location: Cell junction synapse. Golgi apparatus . By similarity. Domain: The A region binds phospholipids with a preference for negatively charged species . By similarity. Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles . By similarity. Sequence similarities: Belongs to the synapsin family.

size1 :

0.01 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage