Anti-Phospho-Ser62,67 Synapsin I | MyBioSource MBS502116 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser62,67 Synapsin I

catalog :

MBS502116

quantity :

0.1 ml

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat, cow

application :

western blot

product information

catalog number :

MBS502116

products type :

Antibody

products full name :

Anti-Phospho-Ser62,67 Synapsin I

products short name :

Synapsin (Ser62,67)

other names :

synapsin-1; Synapsin-1; synapsin-1; Synapsin I

products gene name :

SYN1

other gene names :

SYN1; SYN1

uniprot entry name :

SYN1_BOVIN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

706

specificity :

Specific for ~78k synapsin I doublet phosphorylated at Ser62,67. Immunolabeling of the synapsin I band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

Store at -20 degree C; stable for at least one year

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser62,67 of synapsin I. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Mouse, and bovine . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse, and bovine tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987; Jovanovic et al., 1996).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Jovanovic JN, Benfenati, F, Siow YL, Sihra TS, Sanghera JS, Pelech SL, Greengard P,Czernik AJ (1996) Neurotrophins stimulate phosphorylation of Synapsin I by MAP kinase and regulate Synapsin I-actin interactions. Neurobiology 93:3679-3683. Czernik AJ, Pang DT, Greengard P (1987) Amino acid sequences surrounding the cAMP-dependent and calcium/calmodulin-dependent phosphorylation sites in rat and bovine synapsin I. Proc Natl Acad Sci (USA) 84:7518-7522. Feng J, Chi P, Blanpied TA, Xu YM, Magarinos AM, Ferreira A, Takahashi RH, Kao HT, McEwen BS, Ryan TA, Augustine GJ, Greengard P (2002) Regulation of neurotransmitter release by synapsin III. J Neurosci 22:4372-4380. Jovanovic JN, Sihra TS, Nairn AC, Hemmings HC, Jr., Greengard P, Czernik AJ (2001) Opposing changes in phosphorylation of specific sites in synapsin I during Ca2+-dependent glutamate release in isolated nerve terminals. J Neurosci 21:7944-7953. Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P (2002) A protein kinase A-dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neurosci 5:431-437.

ncbi gi num :

27806393

ncbi acc num :

NP_776616.1

ncbi gb acc num :

NM_174191.2

uniprot acc num :

P17599

ncbi mol weight :

uniprot summary :

Function: Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level . By similarity. Subunit structure: Homodimer. Interacts with CAPON. Forms a ternary complex with NOS1. Isoform Ibinteracts with PRNP . By similarity. Subcellular location: Cell junction synapse. Golgi apparatus . By similarity. Domain: The A region binds phospholipids with a preference for negatively charged species . By similarity. Post-translational modification: Substrate of at least four different protein kinases. Phosphorylation may play a role in the regulation of synapsin-1 in the nerve terminal.Phosphorylation at Ser-9 dissociates synapsins from synaptic vesicles . By similarity. Sequence similarities: Belongs to the synapsin family.

size :

0.1 ml

price :

270 USD