antibody

Anti-NMDA Receptor, NR2B Subunit N-terminus

MBS502092

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Anti-NMDA Receptor, NR2B Subunit N-terminus

NMDA NR2B Subunit, N-terminus

glutamate receptor ionotropic, NMDA 2B; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor ionotropic, NMDA 2B; NR2B; NMDAR2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B

GRIN2B

Grin2b; Grin2b; GluN2B; GluN2B; NMDAR2B; NR2B

NMDE2_RAT

Polyclonal

Rabbit

Rat

1482

Specific for the ~180k NR2B subunit of the NMDA receptor.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

100 ul antibody vial in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least 1 year

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:500

Antigen: Peptide from the N-terminus of the NR2B subunit of rat NMDA receptor. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2B subunit conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primate. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse and non-human primate based on the fact that these species have 100 % homology with the amino acid sequence used as antigen. Biological Significance: The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned and it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).

Affinity purified rabbit polyclonal antibody

Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Clayton DA, Grosshans DR, Browning MD (2002a) Aging and surface expression of hippocampal NMDA receptors. J Biol Chem 277:14367-14369. Clayton DA, Mesches MH, Alvarez E, Bickford PC, Browning MD (2002b) A hippocampal NR2B deficit can mimic age-related changes in long-term potentiation and spatial learning in the Fischer 344 rat. J Neurosci 22:3628-3637. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33.

6980984

NP_036706.1

NM_012574.1

Q00960

180

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (936445); Alcoholism Pathway (585585); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (936452)

subunit of the NMDA receptor glutamate gated ion channel; displays voltage dependent sensitivity to magnesium, calcium permeability, and glycine regulated activity; may facilitate learning and long term potentiation [RGD, Feb 2006]

Function: NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death . By similarity. Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Found in a complex with GRIN1 and GRIN3B. Interacts with MAGI3. Interacts with HIP1 and Neto1 . By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with DAPK1 . By similarity. Ref.3 Ref.4 Ref.5 Ref.6 Ref.7 Ref.8. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Post-translational modification: Phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity . By similarity. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. [View classification]

0.1 mL

345 USD