antibody

Anti-NMDA Receptor, NR2A Subunit N-terminus

MBS502085

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Anti-NMDA Receptor, NR2A Subunit N-terminus

NMDA NR2A Subunit, N-terminus

glutamate receptor ionotropic, NMDA 2A; Glutamate receptor ionotropic, NMDA 2A; glutamate receptor ionotropic, NMDA 2A; N-methyl D-aspartate receptor subtype 2A; N-methyl-D-aspartate receptor subunit 2A; glutamate [NMDA] receptor subunit epsilon-1; glutamate receptor, ionotropic, N-methyl D-aspartate 2A; Glutamate [NMDA] receptor subunit epsilon-1; N-methyl D-aspartate receptor subtype 2A

GRIN2A

Grin2a; Grin2a; NR2A; GluN2A; NMDAR2A; GluN2A; NMDAR2A; NR2A

NMDE1_RAT

Polyclonal

Rabbit

Rat

1464

Specific for the ~180k NR2A subunit of the NMDA receptor.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

Packaging: 100 ul antibody vial in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least 1 year

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:500

Antigen: Peptide from the N-terminus of the NR2A subunit of rat NMDA receptor. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NR2A subunit conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, mouse. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots rat tissue. It is anticipated that the antibody will also react with bovine, canine, and mouse tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and alpha-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits.

Affinity purified rabbit polyclonal antibody

Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. J Biol Chem 278:11020-11025. Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci 25:571-577. Grosshans DR, Clayton DA, Coultrap SJ, Browning MD (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nat Neurosci 5:27-33. Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neurosci 2:331-338.

140971205

NP_036705.3

NM_012573.3

Q00959

180

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (936445); Alcoholism Pathway (585585); Alcoholism Pathway (587116); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Amyotrophic Lateral Sclerosis (ALS) Pathway (83491); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (936452)

may play a role in synaptic transmission, learning and memory [RGD, Feb 2006]

Function: NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits. Subunit structure: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with AIP1. Found in a complex with GRIN1 and GRIN3B. Interacts with HIP1 and NETO1. Interacts with SNX27 (via PDZ domain); the interaction is required for recycling to the plasma membrane when endocytosed and prevent degradation in lysosomes . By similarity. Interacts with PDZ domains of INADL and DLG4. Interacts with LRFN2. Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein Ref.8. Sequence similarities: Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily. [View classification]

0.1 mL

345 USD