antibody

Anti-Metabotropic Glutamate Receptor 5/1a

MBS502077

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Anti-Metabotropic Glutamate Receptor 5/1a

Metabotropic Glutamate Receptor 5/1a

metabotropic glutamate receptor 5; Metabotropic glutamate receptor 5; metabotropic glutamate receptor 5; metabotropic glutamate receptor 5b; metabotropic glutamate receptor (mGluR5); glutamate receptor, metabotropic 5

GRM5

Grm5; Grm5; mGluR5; mGlur5; Gprc1e; Mglur5; mGluR5

GRM5_RAT

Polyclonal

Rabbit

Rat

1171

Specific for the ~125k monomer and the ~250k dimers of mGluR5 and mGluR1. Immunolabeling is blocked by preadsorption of antibody with the peptide used as antigen to generate the antibody.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

100 ul in 200 mM Tris-Glycine (pH 7.5), 0.1% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections, unpublished observations): 1:500

Antigen: Peptide from the C-terminal region of rat mGluR5 and rat mGluR1a. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Human, mouse. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with human and mouse tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Affinity purified rabbit polyclonal antibody

Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761. Blaabjerg M, Fang LW, Zimmer J, Baskys A (2003) Neuroprotection against NMDA excitotoxicity by group I metabotropic glutamate receptors is associated with reduction of NMDA stimulated currents. Exp Neurol 183:573-580. Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res 1022:12-18. Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592.

8393490

NP_058708.1

NM_017012.1

P31424

125/250

Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (936137); G Alpha (q) Signalling Events Pathway (936142); GPCR Downstream Signaling Pathway (936138); GPCR Ligand Binding Pathway (936108); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198511); Gap Junction Pathway (83464); Gap Junction Pathway (483); Gastrin-CREB Signalling Pathway Via PKC And MAPK (936156)

may play a role in synaptic transmission; may interact with mGluR1, another group I metabotropic glutamate receptor [RGD, Feb 2006]

Function: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity. Ref.1. Subunit structure: Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with SIAH1 and GRASP. Interacts with NCDN. Subcellular location: Cell membrane; Multi-pass membrane protein Ref.1. Tissue specificity: Widely distributed in neuronal cells of the central nervous system. Ref.1. Miscellaneous: Activated by quisqualate > glutamate > ibotenate > trans-1- aminocyclopentyl-1,3-dicarboxylate. Sequence similarities: Belongs to the G-protein coupled receptor 3 family.

0.1 mL

345 USD