antibody

Anti-Metabotropic Glutamate Receptor 2/3

MBS502076

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry

Antibody

Anti-Metabotropic Glutamate Receptor 2/3

Metabotropic Glutamate Receptor 2/3

metabotropic glutamate receptor 2; Metabotropic glutamate receptor 2; metabotropic glutamate receptor 2; mGluR2; glutamate receptor, metabotropic 2

GRM2/3

Grm2; Grm2; Gprc1b; Mglur2; mGluR2

GRM2_RAT

Polyclonal

Rabbit

Rat

872

Specific for the ~110k monomer and the ~220k dimer of mGluR2 and mGluR3 in Western Blot of rat brain. Immunolabeling is blocked by preadsorption of antibody with the C-terminal peptide immunogen.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

100 ul in 200 mM Tris-Glycine (pH 7.5), 0.1% sodium azide. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -80 degree C is recommended, but shorter term storage at -20 degree C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

Western Blot (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:500. WB: 1:1000

Antigen: Peptide from the C-terminal region of rat mGluR2 and rat mGluR3. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse, non-human primate and zebra fish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with bovine, canine, human, mouse, non-human primate and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR2 subunit has been shown to be required for long-term potentiation at the mossy fiber input in the hippocampus (Yokoi et al., 1996).

Affinity purified rabbit polyclonal antibody

Bhattacharya M, Babwah AV, Godin C, Anborgh PH, Dale LB, Poulter MO, Ferguson SSG (2004) Ral and phospholipase D2-dependent pathway for constitutive metabotropic glutamate receptor endocytosis. J Neurosci 24:8752-8761. Francesconi W, Cammalleri M, Sanna PP (2004) The metabotropic glutamate receptor 5 is necessary for late-phase long-term potentiation in the hippocampal CA1 region. Brain Res 1022:12-18. Wilson RI, Nicoll RA (2001) Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature (London) 410:588-592. Yokoi M, Kobayashi K, Manabe T, Takahashi T, Sakaguchi I, Katsuura G, Shigemoto R, Ohishi H, Nomura S, Nakamura K, Nakao K, Katsuki M, Nakanishi S (1996) Impairment of hippocampal mossy fiber LTD in mice lacking mGluR2. Science 273:645-647.

157787064

NP_001099181.1

NM_001105711.1

P31421

110/220

Class C/3 (Metabotropic Glutamate/pheromone Receptors) Pathway (936137); Cocaine Addiction Pathway (546271); Cocaine Addiction Pathway (546273); G Alpha (i) Signalling Events Pathway (936140); GPCR Downstream Signaling Pathway (936138); GPCR Ligand Binding Pathway (936108); GPCRs, Class C Metabotropic Glutamate, Pheromone Pathway (198511); Glutamatergic Synapse Pathway (213815); Glutamatergic Synapse Pathway (213811); Neuroactive Ligand-receptor Interaction Pathway (83445)

mediates synaptic transmission; may be involved in brain microvascular function [RGD, Feb 2006]

Function: G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization. Subunit structure: Interacts with HTR2A . By similarity. Interacts with GRASP. Ref.2. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction synapse . By similarity. Cell projection dendrite . By similarity. Tissue specificity: Is widely distributed in the CNS and prominent expression is seen in Golgi cells of the cerebellum and some particular neuronal cells in other brain regions. Sequence similarities: Belongs to the G-protein coupled receptor 3 family.

0.1 mL

345 USD