Anti-Phospho-Ser152,156 MARCKS | MyBioSource MBS502071 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser152,156 MARCKS

catalog :

MBS502071

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502071

products type :

Antibody

products full name :

Anti-Phospho-Ser152,156 MARCKS

products short name :

MARCKS (Ser152,156)

other names :

Myristoylated alanine-rich C-kinase substrate; Myristoylated alanine-rich C-kinase substrate; myristoylated alanine-rich C-kinase substrate; protein kinase C substrate 80 kDa protein; Myristoylated alanine-rich protein kinase C substrate; myristoylated alanine rich protein kinase C substrate; Protein kinase C substrate 80 kDa protein

products gene name :

MARCKS

other gene names :

Marcks; Marcks; KINC; Macs; Macs; MARCKS

uniprot entry name :

MARCS_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

309

specificity :

Specific for the ~87k MARCKS protein phosphorylated at Ser152 and Ser156 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser152,156 of MARCKS. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser152/156 conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine,chicken, human, mouse, Xenopus and zebra fish . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, chicken, human, mouse, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) is a major substrate for phosphorylation by protein kinase C (PKC) (Ouimet et al., 1990). The phosphorylation of Ser152/156 can be used as a measure of PKC activation although these sites are also phosphorylated by PRK1 (Palmer et al., 1996) MARCKS is a member of a family of calmodulin binding proteins and phosphorylation of Ser152/156 modulates the binding of MARCKS to calmodulin (Verghese et al., 1994).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Ouimet CC, Wang JKT, Walaas SI, Albert KA, Greengard P (1990) Localization of the MARCKS (87 kDa) protein, a major specific substrate for protein kinase C, in rat brain. J Neurosci 10:1683-1698. Palmer RH, Schönwasser DC, Rahman D, Pappin DJC, Herget T, Parker PJ (1996) PRK1 phosphorylates MARCKS at the PKC sites: Serine 152, serine 156 and serine 163. FEBS Lett 378:281-285. Verghese GM, Johnson JD, Vasulka C, Haupt DM, Stumpo DJ, Blackshear PJ (1994) Protein kinase C-mediated phosphorylation and calmodulin binding of recombinant myristoylated alanine-rich C kinase substrate (MARCKS) and MARCKS-related protein. J Biol Chem 269:9361-9367.

ncbi gi num :

266495

ncbi acc num :

P30009.2

uniprot acc num :

P30009

ncbi mol weight :

ncbi pathways :

Fc Gamma R-mediated Phagocytosis Pathway (114013); Fc Gamma R-mediated Phagocytosis Pathway (106590); MicroRNAs In Cancer Pathway (852719); MicroRNAs In Cancer Pathway (852928)

ncbi summary :

protein kinase C (PKC) substrate and scaffolding protein [RGD, Feb 2006]

uniprot summary :

Function: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. Subcellular location: Cytoplasm cytoskeleton . Probable. Membrane; Lipid-anchor . By similarity. Tissue specificity: Highest levels found in spleen and brain. Intermediate levels seen in thymus, ovary, lung and heart. Very low levels seen in kidney, skeletal muscle and liver. Ref.3. Post-translational modification: Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity. Sequence similarities: Belongs to the MARCKS family.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage