antibody

Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I

MBS502069

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

phosphorylated

human, rat

western blot, immunohistochemistry

Antibody

Anti-Phospho-Ser535,539 Interferon-alpha Receptor, Type I, Subunit I

Interferon-a Receptor, Type I, Subunit I (Ser535,539)

interferon alpha/beta receptor 1; Interferon alpha/beta receptor 1; interferon alpha/beta receptor 1; CRF2-1; IFN-R-1; IFN-alpha/beta receptor 1; interferon-beta receptor 1; beta-type antiviral protein; alpha-type antiviral protein; type I interferon receptor 1; cytokine receptor class-II member 1; cytokine receptor family 2 member 1; interferon-alpha/beta receptor alpha chain; interferon (alpha, beta and omega) receptor 1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; CRF2-1; Type I interferon receptor 1

IFNAR1

IFNAR1; IFNAR1; AVP; IFRC; IFNAR; IFNBR; IFN-alpha-REC; IFNAR; IFN-R-1; IFN-alpha/beta receptor 1; CRF2-1

INAR1_HUMAN

Polyclonal

Rabbit

Rat

557

Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells; the mature form is ~110 - 130k.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser535,539 of human interferon-alpha receptor, type I, subunit I (IFNAR1). Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH. Immunogen Species: Human

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse, non-human primates and sheep. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, non-human primates and sheep based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001; Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-alpha Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003).

Affinity purified rabbit polyclonal antibody

Biron CA (2001) Interferons alpha and beta as immune regulators--a new look. Immunity 14:661-664. Kirkwood J (2002) Cancer immunotherapy: the interferon-alpha experience. Semin Oncol 29:18-26. Kumar KG, Tang W, Ravindranath AK, Clark WA, Croze E, Fuchs SY (2003) SCF(HOS) ubiquitin ligase mediates the ligand-induced down-regulation of the interferon-alpha receptor. EMBO J 22:5480-5490. K. G. Suresh Kumar, John J. Krolewski, and Serge Y. Fuchs (2004) Phosphorylation and Specific Ubiquitin Acceptor Sites Are Required for Ubiquitination and Degradation of the IFNAR1 Subunit of Type I Interferon Receptor. J. Biol. Chem., Nov 2004; 279: 46614 - 46620.

46488932

NP_000620.2

NM_000629.2

P17181

110-130

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Hepatitis B Pathway (694606); Hepatitis C Pathway (173973); Hepatitis C Pathway (173907); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Immune System Pathway (106386)

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor. [provided by RefSeq, Jul 2008]

Function: Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves. Subunit structure: Heterodimer with IFNAR2; in presence of interferon alpha and beta ligands, the heterodimer forms the type I interferon receptor. Interacts with STAT1 and STAT2; the interaction requires its phosphorylation at Tyr-466. Interacts with IFNAR2. Ref.11 Ref.12. Subcellular location: Isoform 1: Membrane; Single-pass type I membrane protein. Tissue specificity: IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266B1. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R. Ref.9. Post-translational modification: Phosphorylated on tyrosine residues by TYK2 tyrosine kinase. Ref.10Palmitoylation at Cys-463 is required for the activation of STAT1 and STAT2. Ref.14. Sequence similarities: Belongs to the type II cytokine receptor family.Contains 4 fibronectin type-III domains. Sequence caution: The sequence AAH02590.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

0.1 mL

345 USD