antibody

Anti-Phospho-Ser9 GSK3beta

MBS502067

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-Phospho-Ser9 GSK3beta

Glycogen synthase kinase 3 B (GSK3B) (Ser9)

glycogen synthase kinase-3 beta; Glycogen synthase kinase-3 beta; glycogen synthase kinase-3 beta; FA; factor A; GSK-3 beta; serine/threonine-protein kinase GSK3B; glycogen synthase kinase 3 beta; Factor A; FA; Serine/threonine-protein kinase GSK3B

GSK3B

Gsk3b; Gsk3b; GSK-3 beta; FA

GSK3B_RAT

Polyclonal

Rabbit

Rat

420

Specific for the ~46k GSK3beta protein phosphorylated at Ser9. Also weakly labels the ~51k GSK3alpha band due to the high degree of homology between the 2 subunits. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser9 of GSK3beta. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser9 conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Human, mouse, chicken, bovine, canine, non-human primate, Zebrafish and Xenopus. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with human, mouse, chicken, bovine, canine, non-human primate, Zebra fish and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Glycogen synthase kinase 3 (GSK3) is a serine/threonine kinase that is involved in the regulation of many signaling pathways. To date, 2 isoforms have been identified: GSK3alpha and GSK3beta. Specifically, GSK3beta has been shown to play a key inhibitory role in both the insulin and Wnt signaling pathways (Papkoff and Aikawa 1998). It has been suggested that Ser9 phosphorylation underlies the inhibition of GSK3beta by insulin (Sutherland et al., 1993).

Affinity purified rabbit polyclonal antibody

Sutherland C, Leighton IA, Cohen P (1993) Inactivation of glycogen synthase kinase 3 beta by phosphorylation: new kinase connections in insulin and growth factor signaling. Biochem J. Nov 15; 296 (Pt. 1):15-9. Papkoff J, Aikawa M (1998) WNT-1 and HGF regulate GSK3b activity and beta catenin signaling in mammary epithelial cells. Biochem Biophys Res Commun. Jun 29;247(3):851-8.

14091770

NP_114469.1

NM_032080.1

P18266

46

AKT Phosphorylates Targets In The Cytosol Pathway (935977); Adaptive Immune System Pathway (936651); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Axon Guidance Pathway (83457); Axon Guidance Pathway (476); Axon Guidance Pathway (936266); B Cell Receptor Signaling Pathway (198486); B Cell Receptor Signaling Pathway (83473); B Cell Receptor Signaling Pathway (492)

mediates Par6-atypical protein kinase C (aPKC) complex regulation of cell polarity; may induce apoptosis [RGD, Feb 2006]

Function: Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates NFATC1/NFATC on conserved serine residues promoting NFATC1/NFATC nuclear export, shutting off NFATC1/NFATC gene regulation, and thereby opposing the action of calcineurin. Phosphorylates MAPT/TAU on 'Thr-548', decreasing significantly MAPT/TAU ability to bind and stabilize microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Phosphorylates MACF1, inhibiting its binding to microtubules which is critical for its role in bulge stem cell migration and skin wound repair. Probably regulates NF-kappa-B (NFKB1) at the transcriptional level and is required for the NF-kappa-B-mediated anti-apoptotic response to TNF-alpha (TNF/TNFA). Negatively regulates replication in pancreatic beta-cells, resulting in apoptosis, loss of beta-cells. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation. Phosphorylates MUC1 in breast cancer cells, decreasing the interaction of MUC1 with CTNNB1/beta-catenin. Is necessary for the establishment of neuronal polarity and axon outgrowth. Phosphorylates MARK2, leading to inhibit its activity. Phosphorylates SIK1 at 'Thr-182', leading to sustain its activity. Phosphorylates ZC3HAV1 which enhances its antiviral activity. Phosphorylates SFPQ upon T-cell activation. Phosphorylates SNAI1, leading to its BTRC-triggered ubiquitination and proteasomal degradation. Phosphorylates NR1D1 st 'Ser-55' and 'Ser-59' and stabilizes it by protecting it from proteasomal degradation. Ref.4 Ref.6 Ref.7. Catalytic activity: ATP + [tau protein] = ADP + [tau protein] phosphate.ATP + a protein = ADP + a phosphoprotein. Enzyme regulation: Activated by phosphorylation at Tyr-216. In response to insulin, inhibited by phosphorylation at Ser-9 by PKB/AKT1; phosphorylation at this site causes a conformational change, preventing access of substrates to the active site. Inhibited by lithium. Subunit structure: Monomer. Interacts with DAB2IP (via C2 domain); the interaction stimulates GSK3B kinase activation. Interacts (via C2 domain) with PPP2CA . By similarity. Interacts with ARRB2, AXIN1, CABYR, DISC1, MMP2, MUC1, NIN, PRUNE and ZBED3 . By similarity. Interacts with AXIN1; the interaction mediates hyperphosphorylation of CTNNB1 leading to its ubiquitination and destruction. Interacts with and phosphorylates SNAI1. Interacts with DNM1L (via a C-terminal domain) . By similarity. Found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B. Interacts with SGK3 . By similarity. Ref.4 Ref.5. Subcellular location: Cytoplasm . By similarity. Nucleus . By similarity. Membrane. Cell membrane . By similarity. Note: The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosphorylated form to the cell membrane . By similarity. Ref.5. Post-translational modification: Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 and RPS6KA3 protein kinases phosphorylate and desactivate GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216 . By similarity. Ref.3Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity . By similarity. Miscellaneous: Simultaneous silencing of GSK3A and GSK3B by RNAi stimulates replication and promotes survival of INS-1E pancreatic beta cells (Ref.6). Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.Contains 1 protein kinase domain.

0.1 mL

345 USD