Anti-Glycine Receptor | MyBioSource MBS502066 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Glycine Receptor

catalog :

MBS502066

quantity :

0.2 mg

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502066

products type :

Antibody

products full name :

Anti-Glycine Receptor

products short name :

Glycine Receptor

other names :

glycine receptor subunit alpha-1; Glycine receptor subunit alpha-1; glycine receptor subunit alpha-1; glycine receptor 48 kDa subunit; glycine receptor, alpha 1 subunit; glycine receptor strychnine-binding subunit; glycine receptor, alpha 1; Glycine receptor 48 kDa subunit; Glycine receptor strychnine-binding subunit

products gene name :

GLRA1

other gene names :

Glra1; Glra1; GLYRA1; Glyr

uniprot entry name :

GLRA1_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Human, mouse, rat

sequence length :

449

specificity :

Specific for the ~48k alpha1- and alpha2-subunits of the glycine receptor in Western blots of rat spinal cord and brain stem and in cell extracts. Immunolabeling blocked by preadsorption of antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.)

form :

200 ug antibody vial; lyophilized from 5 mM ammonium bicarbonate. The antibody should be reconstituted in 50 ul phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution the antibody s

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC (frozen sections; unpublished observations): 1:1000

other info1 :

Antigen: Peptide from the N-terminus region of the alpha1-subunit of rat glycine receptor. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Immunogen Species: Rat

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in human, mouse and rat tissues. Biological Significance: Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Beckstead MJ, Phelan R, Mihic SJ (2001) Antagonism of inhalant and volatile anesthetic enhancement of glycine receptor function. J Biol Chem 276:24959-24964. Chatterton JE, Awobuluyi M, Premkumar LS, Takahashi H, Talantova M, Shin Y, Cui JK, Tu SC, Kevin ASK, Nakanishi N, Tong G, Lipton SA, Zhang DX (2002) Excitatory glycine receptors containing the NR3 family of NMDA receptor subunits. Nature (London) 415:793-798. Craig AM, Lichtman JW (2001) Getting a bead on receptor movements. Nat Neurosci 4:219-220. Fischer F, Kneussel M, Tintrup H, Haverkamp S, Rauen T, Betz H, Wässle H (2000) Reduced synaptic clustering of GABA and glycine receptors in the retina of the gephyrin null mutant mouse. J Comp Neurol 427:634-648. Mihic SJ, Ye Q, Wick MJ, Koltchine VV, Krasowski MD, Finn SE, Mascia MP, Valenzuela CF, Hanson KK, Greenblatt EP, Harris RA, Harrison NL (1997) Sites of alcohol and volatile anaesthetic action on GABAA and glycine receptors. Nature (London) 389:385-389.

ncbi gi num :

6980950

ncbi acc num :

NP_037265.1

ncbi gb acc num :

NM_013133.1

uniprot acc num :

P07727

ncbi mol weight :

ncbi pathways :

Ion Channel Transport Pathway (935915); Ligand-gated Ion Channel Transport Pathway (935917); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462); Transmembrane Transport Of Small Molecules Pathway (935871)

ncbi summary :

glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]

uniprot summary :

Function: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing). Subunit structure: Pentamer composed of three alpha and two beta subunits. Homopentamers of alpha subunits also form functional receptors. Subcellular location: Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Miscellaneous: The alpha subunit binds strychnine. Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Glycine receptor (TC 1.A.9.3) subfamily. GLRA1 sub-subfamily. [View classification]

size1 :

0.2 mg

price1 :

345 USD

more info or order :

MyBioSource product webpage