antibody

Anti-Phospho-Ser41 Gap-43

MBS502060

0.1 ml

270 USD

polyclonal

rabbit

nonconjugated

phosphorylated

Antibody

Anti-Phospho-Ser41 Gap-43

Gap-43 (Ser41)

neuromodulin; Neuromodulin; neuromodulin; protein F1; growth-associated protein 43; axonal membrane protein GAP-43; growth accentuating protein 43; brain abundant, membrane attached signal protein 2; growth associated protein 43; Axonal membrane protein GAP-43; Growth-associated protein 43; Protein F1

GAP43

Gap43; Gap43; Basp2

NEUM_RAT

Polyclonal

Rabbit

Rat

226

Specific for the ~50k Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with lambda-Ptase.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser41 of Gap-43. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, finch, human, mouse, non-human primates, Xenopus and zebra fish . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, finch, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect eurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).

Affinity purified rabbit polyclonal antibody

Benowitz LI, Routtenberg A (1997) Gap-43: An intrinsic determinant of neuronal development and plasticit. Trends Neurosci 20:84-91. He, Q, Dent, EW, Meiri, KF (1997) Modulation of actin filament behavior by Gap-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17:3515-3524. Hulo S, Alberi, S, Laux T, Muller D, Caroni P (2002) A point mutant of Gap-43 induces enhanced short-term and long-term hippocampal plasticity. Eur J Neurosci 15:1976-1982. Strittmatter SM, Fankhauser C, Huang PL, Mashimo H, Fishman MC (1995) Neuronal path finding is abnormal in mice lacking the neuronal growth cone protein Gap-43," Cell 80:445-452. Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 - 14444.

8393415

NP_058891.1

NM_017195.3

P07936

50

Spinal Cord Injury Pathway 739006

may play a role in neurite outgrowth and nerve regeneration [RGD, Feb 2006]

Function: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction . By similarity. Subunit structure: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN . By similarity. Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence . By similarity. Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction synapse. Cell projection filopodium membrane; Peripheral membrane protein . By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes. Post-translational modification: Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2 . By similarity.Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Ref.7 Ref.8 Ref.9. Sequence similarities: Belongs to the neuromodulin family.Contains 1 IQ domain.

0.1 ml

270 USD