antibody

Anti-GABAA Receptor, beta2-Subunit

MBS502051

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-GABAA Receptor, beta2-Subunit

GABAA Receptor beta2

gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit beta-2; gamma-aminobutyric acid receptor subunit beta-2; GABA(A) receptor subunit beta-2; Gamma-aminobutyric acid receptor beta 2; gamma-aminobutyric acid receptor, subunit beta 2; gamma-aminobutyric acid (GABA-A) receptor, subunit beta 2; gamma-aminobutyric acid (GABA) A receptor, beta 2; GABA(A) receptor subunit beta-2

GABRB2

Gabrb2; Gabrb2; GARB2; Gabrb-2

GBRB2_RAT

Polyclonal

Rabbit

Rat

474

Specific for the ~55k beta2-subunit of the GABAA receptor in Western blots.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Fusion protein from the cytoplasmic loop of the beta2-subunit of rat GABAA receptor. Immunogen Information: Fusion protein from the cytoplasmic loop of the beta 2 subunit. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Canine,human, mouse non-human primate. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, human, mouse and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Affinity purified rabbit polyclonal antibody

McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592. McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480. Pöltl A, Hauer B, Fuchs K, Tretter V, Sieghart W (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455.

6978869

NP_037089.1

NM_012957.2

P63138

55

GABA A Receptor Activation Pathway (936461); GABA Receptor Activation Pathway (936460); GABAergic Synapse Pathway (377244); GABAergic Synapse Pathway (377129); Ion Channel Transport Pathway (935915); Ligand-gated Ion Channel Transport Pathway (935917); Morphine Addiction Pathway (552694); Morphine Addiction Pathway (554808); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462)

subunit of GABA-A receptors, which induce inhibitory postsynaptic currents; mediates desentization [RGD, Feb 2006]

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1. Interacts with KCTD8, KCTD12 and KCTD16; this interaction determines the pharmacology and kinetics of the receptor response, the KCTD proteins markedly accelerating the GABA-B response, although to different extents. Ref.3 Ref.4. Subcellular location: Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB2 sub-subfamily. [View classification]

0.1 mL

345 USD