antibody

Anti-GABAA Receptor, alpha6-Subunit

MBS502048

0.05 mL

300 USD

polyclonal

rabbit

nonconjugated

mouse, rat

Antibody

Anti-GABAA Receptor, alpha6-Subunit

GABAA Receptor a 6

gamma-aminobutyric acid receptor subunit alpha-6; Gamma-aminobutyric acid receptor subunit alpha-6; gamma-aminobutyric acid receptor subunit alpha-6; GABA-A receptor alpha-6 subunit; GABA(A) receptor subunit alpha-6; gamma-aminobutyric acid A receptor, alpha 6; gamma-aminobutyric acid A receptor, alpha 6; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 6; gamma-aminobutyric acid (GABA) A receptor, alpha 6; GABA(A) receptor subunit alpha-6

GABRA6

Gabra6; Gabra6; Gabra-6

GBRA6_RAT

Polyclonal

Rabbit

Rat, mouse

453

Labels the ~57k alpha6-subunit of the GABAA receptor in Western blots of rat brain extracts.

Neat Serum (Unpurified neat serum.)

50 ul neat serum. Adequate material to conduct 10 mini-Western blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Peptide representing a sequence that is specific for the alpha6-subunit of rat GABAA receptor. Peptide antigen has no homology with any other GABAA R-subunit. Immunogen Information: Synthetic peptide corresponding to amino acid residues specific to the alpha 6 subunit conjugated to KLH. Immunogen Species: Rat

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).

Rabbit polyclonal antibody

Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor beta-subunits. Mol Cell Neurosci 22:87-97. McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592. Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.

11177872

NP_068613.1

NM_021841.1

P30191

57

GABA A Receptor Activation Pathway (936461); GABA Receptor Activation Pathway (936460); GABAergic Synapse Pathway (377244); GABAergic Synapse Pathway (377129); Ion Channel Transport Pathway (935915); Ligand-gated Ion Channel Transport Pathway (935917); Morphine Addiction Pathway (552694); Morphine Addiction Pathway (554808); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462)

GABAA receptor subunit; part of a cerebellar receptor subtype, selective for antagonist of alcohol-induced motor incoordination and ataxia [RGD, Feb 2006]

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Subunit structure: Binds UBQLN1 . By similarity. Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Subcellular location: Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Tissue specificity: Only found in cerebellar granule cells. Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA6 sub-subfamily. [View classification]

0.05 mL

300 USD