Anti-GABAA Receptor, alpha4-Subunit | MyBioSource MBS502045 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-GABAA Receptor, alpha4-Subunit

catalog :

MBS502045

quantity :

0.1 ml

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502045

products type :

Antibody

products full name :

Anti-GABAA Receptor, alpha4-Subunit

products short name :

GABAA Receptor a 4,

other names :

gamma-aminobutyric acid receptor subunit alpha-4; Gamma-aminobutyric acid receptor subunit alpha-4; gamma-aminobutyric acid receptor subunit alpha-4; GABA-A receptor alpha-4 subunit; GABA(A) receptor subunit alpha-4; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 4; gamma-aminobutyric acid (GABA) A receptor, alpha 4; GABA(A) receptor subunit alpha-4

products gene name :

GABRA4

other gene names :

Gabra4; Gabra4

uniprot entry name :

GBRA4_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

552

specificity :

Specific for the ~64k alpha4-subunit of the GABAA receptor in Western blots.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Fusion protein from the cytoplasmic loop of the alpha4-subunit of rat GABAA receptor. Immunogen Information: Fusion protein from the cytoplasmic loop of the alpha 4 subunit. Immunogen Species: Rat

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Brandon NJ, Jovanovic JN, Colledge M, Kittler JT, Brandon JM, Scott JD, Moss SJ (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor beta-subunits. Mol Cell Neurosci 22:87-97. McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592. Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629. Whiting PJ, Bonnert TP, McKernan RM, Farrar S, Le Bourdellès B, Heavens RP, Smith DW, Hewson L, Rigby MR, Sirinathsinghji DJS, Thompson SA, Wafford KA (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653.

ncbi gi num :

94400793

ncbi acc num :

NP_542154.3

ncbi gb acc num :

NM_080587.3

uniprot acc num :

P28471

ncbi mol weight :

ncbi pathways :

GABAergic Synapse Pathway 377244!!GABAergic Synapse Pathway 377129!!Morphine Addiction Pathway 552694!!Morphine Addiction Pathway 554808!!Neuroactive Ligand-receptor Interaction Pathway 83445!!Neuroactive Ligand-receptor Interaction Pathway 462!!Nicotine Addiction Pathway 583273!!Nicotine Addiction Pathway 584676!!Retrograde Endocannabinoid Signaling Pathway 537882!!Retrograde Endocannabinoid Signaling Pathway 539806

ncbi summary :

alpha 4 subunit of the GABA-A receptor which binds gamma-aminobutyric acid; involved in mood and anxiety [RGD, Feb 2006]

uniprot summary :

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Subcellular location: Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-subfamily. [View classification]

size :

0.1 ml

price :

270 USD

more info or order :

MyBioSource product webpage