antibody

Anti-GABAA Receptor, alpha1-Subunit

MBS502042

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

mouse, rat

Antibody

Anti-GABAA Receptor, alpha1-Subunit

GABAA Receptor a 1

gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit alpha-1; gamma-aminobutyric acid receptor subunit alpha-1; GABA(A) receptor subunit alpha-1; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; GABA(A) receptor subunit alpha-1

GABRA1

Gabra1; Gabra1; Gabra-1

GBRA1_RAT

Polyclonal

Rabbit

Mouse rat

455

Specific for the ~51k alpha1-subunit of the GABA A -R in Western blots of rat brain membrane fractions. Also recognizes the Alpha1-subunit of the GABA A -R in bovine, human and mouse brain extracts.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -80 degree C is recommended, but shorter term storage at -20 degree C is also acceptale as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20 degree C.

Western Blot (WB), Dot blots (DB). Suitability for Immunohistochemistry (IHC) not yet determined.

Quality Control: Dot blots and Western blots performed on each lot. DB: 1:1000. WB: 1:1000

Antigen: Fusion protein from the cytoplasmic loop of the alpha1-subunit of rat GABAA receptor. Immunogen Information: Fusion protein from the cytoplasmic loop of the alpha 1 subunit. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse non-human primate. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with bovine, canine, human, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Affinity purified rabbit polyclonal antibody

McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor alpha1-subtype. Nature Neurosci 3:587-592. Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing alpha1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. Ogris W, Pöltl A, Hauer B, Ernst M, Oberto A, Wulff P, Höger H, Wisden W, Sieghart W (2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor gamma2-subunit. Biochem Pharmacol 68:1621-1629. Mridula Rewal, Rachel Jurd, T. Michael Gill, Dao-Yao He, Dorit Ron, and Patricia H. Janak (2009) 4-Containing GABAA Receptors in the Nucleus Accumbens Mediate Moderate Intake of Alcohol J. Neurosci., 29: 543 - 549. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480.

34328532

NP_899155.1

NM_183326.2

P62813

51

GABA A Receptor Activation Pathway (936461); GABA Receptor Activation Pathway (936460); GABAergic Synapse Pathway (377244); GABAergic Synapse Pathway (377129); Ion Channel Transport Pathway (935915); Ligand-gated Ion Channel Transport Pathway (935917); Morphine Addiction Pathway (552694); Morphine Addiction Pathway (554808); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462)

subunit of GABAA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain [RGD, Feb 2006]

Function: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. Subunit structure: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1. Interacts with TRAK1 . By similarity. Ref.8. Subcellular location: Cell junction synapse postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Sequence similarities: Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily. [View classification]

0.1 mL

345 USD