antibody

Anti-ERK/MAPK

MBS502034

0.1 ml

270 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-ERK/MAPK

ERK/MAPK

mitogen-activated protein kinase 1; Mitogen-activated protein kinase 1; mitogen-activated protein kinase 1; ERT1; ERK-2; MAPK 1; MAPK 2; p42-MAPK; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; mitogen-activated protein kinase 2; extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2; mitogen activated protein kinase 1; ERT1; Extracellular signal-regulated kinase 2; ERK-2; MAP kinase isoform p42; p42-MAPK; Mitogen-activated protein kinase 2

MAPK1/2

Mapk1; Mapk1; Erk2; Erk2; Mapk; Prkm1; MAP kinase 1; MAPK 1; ERK-2; p42-MAPK; MAP kinase 2; MAPK 2

MK01_RAT

Polyclonal

Rabbit

Rat

358

Specific for the ~42k - 44k ERK/MAPK protein. Immunolabeling is blocked by the peptide used as antigen.

Affinity Purified (Prepared from rabbit serum by affinity purification)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Peptide corresponding to amino acid residues from the C-terminus of rat ERK/MAPK. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Mouse. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with mouse tissue based on the fact that this species has 100% homology with the amino acid sequence used as antigen. Biological Significance: Extracellular-Signal Regulated Kinase/Mitogen-Activated Protein Kinase (ERK/MAPK) is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory (Adams and Sweatt, 2002; Ahn, 1993; Tanoue and Nishida, 2003; Johnson and Lapadat, 2002). The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204 (Ahn, 1993).

Affinity purified rabbit polyclonal antibody

Adams JP, Sweatt JD (2002) Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 42:135-163. Ahn, NG (1993) The MAP kinase cascade. Discovery of a new signal transduction pathway. Mol Cell Biochem 127-128:201-209. Johnson GL, Lapadat R (2002) Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912. Tanoue TJ, Nishida, E (2003) Molecular recognitions in the MAP kinase cascades. Cellular Signaling 15:455-462.

16758698

NP_446294.1

NM_053842.1

P63086

42/44

ARMS-mediated Activation Pathway 936054!!Activated TLR4 Signalling Pathway 936709!!Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway 936445!!Activation Of The AP-1 Family Of Transcription Factors Pathway 936691!!Acute Myeloid Leukemia Pathway 83505!!Acute Myeloid Leukemia Pathway 529!!Adherens Junction Pathway 83462!!Adherens Junction Pathway 481!!Adrenergic Signaling In Cardiomyocytes Pathway 908278!!Adrenergic Signaling In Cardiomyocytes Pathway 909696

kinase involved in intracellular signaling; component of Mapk signaling pathway [RGD, Feb 2006]

Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation . By similarity. Ref.21Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity . By similarity. Ref.21. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium . By similarity. Enzyme regulation: Phosphorylated by MAP2K1/MEK1 and MAP2K2/MEK2 on Thr-183 and Tyr-185 in response to external stimuli like insulin or NGF. Both phosphorylations are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates. Phosphorylation on Ser-27 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. Dephosphorylated and inactivated by DUSP3, DUSP6 and DUSP9. Inactivated by pyrimidylpyrrole inhibitors. Subunit structure: Binds both upstream activators and downstream substrates in multimolecular complexes. Interacts with ADAM15, ARHGEF2, ARRB2, DAPK1 (via death domain), HSF4, IER3, IPO7, MKNK2, DUSP6, MORG1, NISCH, PEA15, SGK1, and isoform 1of NEK2 . By similarity. Interacts (phosphorylated form) with CAV2 ('Tyr-19'-phosphorylated form); the interaction, promoted by insulin, leads to nuclear location and MAPK1 activation . By similarity. MKNK2 isoform 1binding prevents from dephosphorylation and inactivation . By similarity. Interacts with DCC. The phosphorylated form interacts with PML . By similarity. Ref.6 Ref.8 Ref.9 Ref.17 Ref.21. Subcellular location: Cytoplasm cytoskeleton spindle . By similarity. Nucleus . By similarity. Cytoplasm cytoskeleton microtubule organizing center centrosome . By similarity. Cytoplasm . By similarity. Note: Associated with the spindle during prometaphase and metaphase . By similarity. PEA15-binding and phosphorylated DAPK1 promote its cytoplasmic retention . By similarity. Phosphorylation at Ser- 244 and Ser-246 as well as autophosphorylation at Thr-188 promote nuclear localization . By similarity. Tissue specificity: Highest levels within the nervous system, expressed in different tissues, mostly in muscle, thymus and heart. Developmental stage: Increased expression during development. Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. Post-translational modification: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme. Phosphorylated upon FLT3 and KIT signaling. Ligand-activated ALK induces tyrosine phosphorylation . By similarity. Dephosphorylated by PTPRJ at Tyr-185 . By similarity. Autophosphorylated on threonine and tyrosine residues in vitro, which correlates with a slow and low level of activation. Phosphorylation on Ser-27 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2 . By similarity. Ref.4 Ref.5ISGylated . By similarity. Sequence similarities: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.

0.1 ml

270 USD