antibody

Anti-Phospho-Ser383 Elk-1

MBS502030

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

phosphorylated

human, rat

Antibody

Anti-Phospho-Ser383 Elk-1

Elk-1 (Ser383)

ETS domain-containing protein Elk-1 isoform a; ETS domain-containing protein Elk-1; ETS domain-containing protein Elk-1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene; ELK1, member of ETS oncogene family

ELK1

ELK1; ELK1

ELK1_HUMAN

Polyclonal

Rabbit

Rat

428

Specific for ~46k Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser383 of human Elk-1. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH. Immunogen Species: Human

Reactivity Assumed Based on 100% Sequence Homology: Canine, human, mouse non-human primate zebra fish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with canine, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).

Affinity purified rabbit polyclonal antibody

Hill CS, Treisman R (1995) Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell 80:199-211. Li QJ, Yang SH, Maeda Y, Sladek FM, Sharrocks AD. Martins-Green M (2003) MAP kinase phosphorylationdependent activation of Elk-1 leads to activation of the co-activator p300. EMBO J 22:281-291. Thiels E, Kanterewicz BI, Norman ED, Trzaskos, JM, Klann E (2002) Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1. J Neurosci 22:2054-2062.

166362732

NP_001107595.1

NM_001114123.2

P19419

46

Activated TLR4 Signalling Pathway (106400); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); B Cell Receptor Signaling Pathway (198909); BCR Signaling Pathway (138058); BDNF Signaling Pathway (712093); Corticotropin-releasing Hormone Pathway (920957); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); EGFR1 Signaling Pathway (198782); ERK/MAPK Targets Pathway (106482); Endometrial Cancer Pathway (83109)

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Function: Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element. Subunit structure: Interacts in its sumoylated form with PIAS2/PIASX which enhances its transcriptional activator activity. Interacts with MAD2L2; the interaction is direct and promotes phosphorylation by the kinases MAPK8 and/or MAPK9. Ref.17 Ref.18. Subcellular location: Nucleus. Tissue specificity: Lung and testis. Post-translational modification: Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Also phosphorylated on Ser-383 by MAPK8 and/or MAKP9. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity. Phosphorylated and activated by CAMK4, MAPK11, MAPK12 and MAPK14. Ref.9 Ref.11 Ref.12 Ref.13 Ref.18. Sequence similarities: Belongs to the ETS family.Contains 1 ETS DNA-binding domain.

0.1 mL

345 USD