Anti-DOPA Decarboxylase | MyBioSource MBS502021 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-DOPA Decarboxylase

catalog :

MBS502021

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, rat, dog, cow

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502021

products type :

Antibody

products full name :

Anti-DOPA Decarboxylase

products short name :

DOPA Decarboxylase, Human

other names :

aromatic-L-amino-acid decarboxylase isoform 1; Aromatic-L-amino-acid decarboxylase; aromatic-L-amino-acid decarboxylase; dopa decarboxylase (aromatic L-amino acid decarboxylase); DOPA decarboxylase

products gene name :

DDC

other gene names :

DDC; DDC; AADC; AADC; AADC; DDC

uniprot entry name :

DDC_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Bovine, canine, guinea pig, human, rabbit, rat and sheep tissues

sequence length :

480

specificity :

Specific for the ~55k DDC protein

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification using an AminoLink Plus column matrix to which purified, recombinant bovine DDC was coupled.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Peptide from N-terminus region of human DOPA decarboxylase (DDC), conjugated to keyhole limpet hemocyanin (KLH). Immunogen Information: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Immunogen Species: Human

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in bovine, canine, guinea pig, human, rabbit, rat and sheep tissues. Biological Significance: DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087. Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593. Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.

ncbi gi num :

4503281

ncbi acc num :

NP_000781.1

ncbi gb acc num :

NM_000790.3

uniprot acc num :

P20711

ncbi mol weight :

ncbi pathways :

Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amine-derived Hormones Pathway (160983); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Biogenic Amine Synthesis Pathway (198793); Catecholamine Biosynthesis Pathway (160984); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Cocaine Addiction Pathway (546258)

ncbi summary :

The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]

uniprot summary :

Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Catalytic activity: L-dopa = dopamine + CO2.5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO2. Cofactor: Pyridoxal phosphate. Ref.10. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. Subunit structure: Homodimer. Ref.10. Involvement in disease: Aromatic L-amino-acid decarboxylase deficiency (AADCD) [MIM:608643]: An inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. It causes developmental and psychomotor delay, poor feeding, lethargy, ptosis, intermittent hypothermia, gastrointestinal disturbances. The onset is early in infancy and inheritance is autosomal recessive.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.3 Ref.11 Ref.14 Ref.15. Sequence similarities: Belongs to the group II decarboxylase family.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage