Anti-DOPA Decarboxylase | MyBioSource MBS502020 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-DOPA Decarboxylase

catalog :

MBS502020

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat, cow

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502020

products type :

Antibody

products full name :

Anti-DOPA Decarboxylase

products short name :

DOPA Decarboxylase, Bovine

other names :

aromatic-L-amino-acid decarboxylase; Aromatic-L-amino-acid decarboxylase; aromatic-L-amino-acid decarboxylase; AADC; aromatic L-amino acid decarboxylase; dopa decarboxylase (aromatic L-amino acid decarboxylase); DOPA decarboxylase

products gene name :

DDC

other gene names :

DDC; DDC; AADC; DDC

uniprot entry name :

DDC_BOVIN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

487

specificity :

Specific for the ~55k DDC protein.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification using an AminoLink Plus column matrix to which purified, recombinant bovine DDC was coupled.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: SDS denatured, recombinant bovine aromatic DOPA decarboxylase (DDC) expressed in E. coli and purified from inclusion bodies. Immunogen Information: SDS denatured, recombinant bovine aromatic DOPA decarboxylase expressed in E. coli and purified from inclusion bodies. Immunogen Species: Bovine

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat tissues. Biological Significance: DOPA decarboxylase (aromatic L-amino acid decarboxylase, AADC; DDC) catalyzes the second reaction in the biosynthesis of catecholamines and serotonin (Waymire and Haycock, 2002; Berry et al., 1996; Haycock et al., 2003). It is also involved in the biosynthesis of trace amines. DDC antibodies can therefore be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Berry MD, Juorio AV, Li XM, Boulton AA (1996) Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem Res 21:1075-1087. Haycock JW, Becker L, Ang L, Furukawa Y, Hornykiewicz O, Kish SJ (2003) Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J Neurochem 87:574-585. Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567. Waymire JC, Haycock JW (2002) Lack of regulation of aromatic L-amino acid decarboxylase in intact bovine chromaffin cells. J Neurochem 81:589-593. Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.

ncbi gi num :

27806901

ncbi acc num :

NP_776332.1

ncbi gb acc num :

NM_173907.2

uniprot acc num :

P27718

ncbi mol weight :

ncbi pathways :

Alcoholism Pathway (585567); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547615); Amphetamine Addiction Pathway (550546); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (390994); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Cocaine Addiction Pathway (546262); Cocaine Addiction Pathway (546273); Dopaminergic Synapse Pathway (469197); Dopaminergic Synapse Pathway (469185)

uniprot summary :

Function: Catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Catalytic activity: L-dopa = dopamine + CO2.5-hydroxy-L-tryptophan = 5-hydroxytryptamine + CO2. Cofactor: Pyridoxal phosphate. Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 2/2. Subunit structure: Homodimer. Sequence similarities: Belongs to the group II decarboxylase family.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage