Anti-Phospho-ThrDARPP-32 | MyBioSource MBS502019 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-ThrDARPP-32

catalog :

MBS502019

quantity :

0.1 mL

price :

345 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

antigen modification :

phosphorylated

reactivity :

rat

application :

western blot

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS502019

products type :

Antibody

products full name :

Anti-Phospho-ThrDARPP-32

products short name :

DARPP-32 (Thr75)

other names :

protein phosphatase 1 regulatory subunit 1B; Protein phosphatase 1 regulatory subunit 1B; protein phosphatase 1 regulatory subunit 1B; neuronal phosphoprotein DARPP-32; dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; protein phosphatase 1, regulatory (inhibitor) subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein

products gene name :

PPP1R1B

other gene names :

Ppp1r1b; Ppp1r1b; Darpp32; Darpp-32

uniprot entry name :

PPR1B_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

205

specificity :

Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by lambda-phosphatase treatment.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr75 of rat DARPP-32. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, and non-human primates. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac (Maldve et al., 2002; Lindskog et al., 2002; Svenningsson et al., 2002).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Bibb JA, Snyder GL, Nishi A, Yan Z, Meijer L, Fienberg AA, Tsai LH, Kwon YT, Girault JA, Czernik AJ, Huganir RL, Hemmings HC, Jr., Nairn AC, Greengard P (1999) Phosphorylation of DARPP-32 by cdk5 modulates dopamine signalling in neurons. Nature (London) 402:669-671. Fienberg, A.A., Hiroi, N., Mermelstein, P.G., Song, W., Snyder, G.L., Nishi, A., Cheramy, A. O'Callaghan, J.P., Miller, D.B., Cole, D.G., Corbett, R., Haile, C.N., Cooper, D,C., Onn, S.P., Grace, A.A., Ouimet, C.C., White, F.G., Hyman, S.E., Surmeier, D.G., Girault, J., Nestler, E.J. and Greengard, P. (1998) DARPP-32: regulator of the efficacy of dopaminergic neurotransmission. Science 281:838-842. Lindskog M, Svenningsson P, Pozzi L, Kim Y, Fienberg AA, Bibb JA, Fredholm BB, Nairn AC, Greengard P, Fisone G (2002) Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine. Nature (London) 418:774-778. Maldve RE, Zhang TA, Ferrani-Kile K, Schreiber SS, Lippmann MJ, Snyder GL, Feinberg AA, Leslie SW, Gonzales RA, Morrisett RA (2002) DARPP-32 and the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens. Nature Neurosci 5:641-648. Svenningsson P, Tzavara ET, Witkin JM, Fienberg AA, Nomikos GG, Greengard P (2002) Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac). Proc Natl Acad Sci USA 99:3182-3187.

ncbi gi num :

61889054

ncbi acc num :

NP_612530.1

ncbi gb acc num :

NM_138521.1

uniprot acc num :

Q6J4I0

ncbi mol weight :

ncbi pathways :

Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Cocaine Addiction Pathway (546271); Cocaine Addiction Pathway (546273); DARPP-32 Events Pathway (936155); Dopaminergic Synapse Pathway (469193); Dopaminergic Synapse Pathway (469185); Opioid Signalling Pathway (936147)

ncbi summary :

potent inhibitor of protein phosphatase 1 (PP1); may play a role in maintaining the levels of excitability of NMDA receptors [RGD, Feb 2006]

uniprot summary :

Function: Inhibitor of protein-phosphatase 1 . By similarity. Subcellular location: Cytoplasm . By similarity. Post-translational modification: Phosphorylation of Thr-34 is required for activity . By similarity.Dopamine- and cyclic AMP-regulated neuronal phosphoprotein . By similarity. Sequence similarities: Belongs to the protein phosphatase inhibitor 1 family.

size1 :

0.1 mL

price1 :

345 USD

more info or order :

MyBioSource product webpage