antibody

Anti-Phospho-Ser133 CREB

MBS502016

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

phosphorylated

western blot, immunohistochemistry

Antibody

Anti-Phospho-Ser133 CREB

CREB (Ser133)

cyclic AMP-responsive element-binding protein 1 isoform B; Cyclic AMP-responsive element-binding protein 1; cyclic AMP-responsive element-binding protein 1; CREB-1; Y protein; cAMP response element binding protein 1; cAMP-responsive element-binding protein 1; cAMP responsive element binding protein 1

CREB1

Creb1; Creb1; Creb; Creb-1; CREB-1

CREB1_RAT

Polyclonal

Rabbit

Rat

341

Specific for the ~45k CREB protein phosphorylated at Ser133. Immunolabeling is blocked by lambda-phosphatase treatment.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Western Blot (WB), Immunohistochemistry (IHC)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: 1:100

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser133 of rat CREB. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser133 conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish . Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse, non-human primate, rat, sheep, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: It is well known that the control of gene expression involves activation of protein kinase cascades that regulate transcription factors within the nucleus (Karin and Hunter, 1995). The cyclic AMP response element binding protein (CREB) is one of the best characterized stimulus-induced transcription factors (Montminy, 1997). This transcription factor is a component of intracellular signaling events that regulate a wide range of biological functions, from spermatogenesis to circadian rhythms and memory (Shaywitz and Greenberg, 1999; Silva et al., 1998). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin-dependent protein kinases (CaMKs) phosphorylate CREB at serine 133 (Ser133), and phosphorylation of Ser133 are required for CREB-mediated transcription (Johannessen et al., 2004; Kornhauser et al., 2002).

Affinity purified rabbit polyclonal antibody

Johannessen M, Delghandi MP, Moens U (2004) What turns CREB on Cellular Signalling 16:1211-1227. Karin M, Hunter T (1995) Transcriptional control by protein phosphorylation: Signal transmission from the cell surface to the nucleus. Curr Biol 5:747-757. Kornhauser JM, Cowan CW, Shaywitz AJ, Dolmetsch RE, Griffith EC, Hu LS, Haddad C, Xia ZG, Greenberg ME (2002) CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 34:221-233. Montminy M (1997) Transcriptional regulation by cyclic AMP. Annu Rev Biochem 66:807-822. Shaywitz AJ, Greenberg ME (1999) CREB: A stimulus-induced transcription factor activated by a diverse array of extracellular signals. Annu Rev Biochem 68:821-861. Silva AJ, Kogan JH, Frankland PW, Kida S (1998) CREB and memory. Annu Rev Neurosci 21:127-148.

13591924

NP_112279.1

NM_031017.1

P15337

45

AKT Phosphorylates Targets In The Nucleus Pathway (935978); Activated TLR4 Signalling Pathway (936709); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (936445); Adaptive Immune System Pathway (936651); Adipogenesis Pathway (198479); Adrenergic Signaling In Cardiomyocytes Pathway (908278); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Alcoholism Pathway (585585); Alcoholism Pathway (587116); Amphetamine Addiction Pathway (547642)

binds the cAMP response element in many gene promoters and regulates transcription [RGD, Feb 2006]

Function: Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-117 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells . By similarity. Subunit structure: Interacts with PPRC1. Binds DNA as a dimer. Interacts, through the bZIP domain, with the coactivators TORC1/CRTC1, TORC2/CRTC2 and TORC3/CRTC3 . By similarity. When phosphorylated on Ser-133, binds CREBBP. Interacts with ARRB1. Interacts (phosphorylated form) with TOX3. Binds to HIPK2 . By similarity. Interacts with SGK1 . By similarity. Interacts with CREBL2; regulates CREB1 phosphorylation, stability and transcriptional activity . By similarity. Subcellular location: Nucleus. Post-translational modification: Phosphorylation of Ser-133 allows CREBBP binding. Stimulated by phosphorylation. Phosphorylation of both Ser-142 and Ser-133 in the SCN regulates the activity of CREB and participate in circadian rhythm generation . By similarity. Phosphorylated upon calcium influx by CaMK4 and CaMK2 on Ser-133. CaMK4 is much more potent than CaMK2 in activating CREB. Phosphorylated by CaMK2 on Ser-142. Phosphorylation of Ser-142 blocks CREB-mediated transcription even when Ser-133 is phosphorylated. Phosphorylated by CaMK1. Phosphorylation of Ser-271 by HIPK2 in response to genotoxic stress promotes CREB1 activity, facilitating the recruitment of the coactivator CBP. Phosphorylated at Ser-133 by RPS6KA3, RPS6KA4 and RPS6KA5 in response to mitogenic or stress stimuli . By similarity. CREBL2 positively regulates phosphorylation at Ser-133 thereby stimulating CREB1 transcriptional activity . By similarity. Ref.3 Ref.4 Ref.5Sumoylated with SUMO1. Sumoylation on Lys-304, but not on Lys-285, is required for nuclear localization of this protein. Sumoylation is enhanced under hypoxia, promoting nuclear localization and stabilization . By similarity. Sequence similarities: Belongs to the bZIP family.Contains 1 bZIP (basic-leucine zipper) domain.Contains 1 KID (kinase-inducible) domain.

0.1 mL

345 USD