antibody

Anti-Phospho-Ser368 Connexin43

MBS502015

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

phosphorylated

Antibody

Anti-Phospho-Ser368 Connexin43

Connexin 43 (Ser368)

gap junction alpha-1 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1; Connexin-43; Cx43; Gap junction 43 kDa heart protein

GJA1

Gja1; Gja1; Cx43; Cxn-43; Cx43

CXA1_RAT

Polyclonal

Rabbit

Rat

382

Specific for the ~43k connexin43 protein phosphorylated at Ser368. Immunolabeling is blocked by lambda-phosphatase treatment.

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least one year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser368 of rat connexin43. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, guinea pig, human, mouse and zebra fish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, guinea pig, human, mouse and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Affinity purified rabbit polyclonal antibody

Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459. Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654. Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066. Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.

6978896

NP_036699.1

NM_012567.2

P08050

43

Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117251); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Calcium Regulation In The Cardiac Cell Pathway (198495); EGFR1 Signaling Pathway (198427); Formation Of Annular Gap Junctions Pathway (935772); Gap Junction Pathway (83464); Gap Junction Pathway (483); Gap Junction Assembly Pathway (935766); Gap Junction Degradation Pathway (935771); Gap Junction Trafficking Pathway (935765)

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

Function: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract . By similarity. Subunit structure: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150. Interacts with CNST and CSNK1D . By similarity. Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts with UBQLN4. Ref.10 Ref.12 Ref.13. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction gap junction Ref.13. Induction: In bladder smooth muscle cells, exhibits night/day variations with low levels during the sleep phase, at circadian time (CT) 4-8 (at protein level). Expression starts to increase around CT12 and forms a plateau during the active phase (CT16-24) (at protein level). Ref.11. Post-translational modification: Contains at least one intramolecular disulfide bond.Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity . By similarity. Ref.9Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2 . By similarity.S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.

0.1 mL

345 USD