antibody

Anti-Connexin43

MBS502014

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-Connexin43

Connexin 43

gap junction alpha-1 protein; Gap junction alpha-1 protein; gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1; Connexin-43; Cx43; Gap junction 43 kDa heart protein

GJA1

Gja1; Gja1; Cx43; Cxn-43; Cx43

CXA1_RAT

Polyclonal

Rabbit

Rat

382

Specific for the ~43k connexin43 protein.

Affinity Purified (Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least one year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: Not recommended

Antigen: Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43. Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Chicken, canine, finch, human, mouse non-human primate, sheep Xenopus and zebra fish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).

Affinity purified rabbit polyclonal antibody

Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459. Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654. Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066. Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.

6978896

NP_036699.1

NM_012567.2

P08050

43

Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117251); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Calcium Regulation In The Cardiac Cell Pathway (198495); EGFR1 Signaling Pathway (198427); Formation Of Annular Gap Junctions Pathway (935772); Gap Junction Pathway (83464); Gap Junction Pathway (483); Gap Junction Assembly Pathway (935766); Gap Junction Degradation Pathway (935771); Gap Junction Trafficking Pathway (935765)

gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]

Function: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract . By similarity. Subunit structure: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150. Interacts with CNST and CSNK1D . By similarity. Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts with UBQLN4. Ref.10 Ref.12 Ref.13. Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction gap junction Ref.13. Induction: In bladder smooth muscle cells, exhibits night/day variations with low levels during the sleep phase, at circadian time (CT) 4-8 (at protein level). Expression starts to increase around CT12 and forms a plateau during the active phase (CT16-24) (at protein level). Ref.11. Post-translational modification: Contains at least one intramolecular disulfide bond.Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity . By similarity. Ref.9Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2 . By similarity.S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.

0.1 mL

345 USD