Anti-Phospho-Ser129 Alpha Synuclein | MyBioSource MBS502004 product information

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product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Phospho-Ser129 Alpha Synuclein

catalog :

MBS502004

quantity :

0.1 ml

price :

270 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

rat

application :

western blot

product information

catalog number :

MBS502004

products type :

Antibody

products full name :

Anti-Phospho-Ser129 Alpha Synuclein

products short name :

Alpha synuclein (ser129)

other names :

alpha-synuclein; Alpha-synuclein; alpha-synuclein; synuclein, alpha (non A4 component of amyloid precursor)

products gene name :

SNCA

other gene names :

Snca; Snca

uniprot entry name :

SYUA_RAT

clonality :

Polyclonal

host :

Rabbit

reactivity :

Rat

sequence length :

140

specificity :

Specific for the ~15 kDa alpha-synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.

purity :

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

Store at -20 degree C; stable for at least one year.

tested application :

Western Blot (WB)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1000

other info1 :

Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser129 of alpha synuclein. Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 conjugated to KLH. Immunogen Species: Rat

other info2 :

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, human, mouse, and non-human primate. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, human, mouse, and non-human primate based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: Alpha-synuclein is a presynaptic neuronal protein that is thought to be involved in the formation of SNARE complexes. Most significantly, aggregated alpha-synuclein is one of the major components found in the Lewy bodies that occur in Parkinson's disease (PD) and other neurodegenerative disorders (Okochi et al., 2000). Mutation of the alpha-synuclein gene is associated with familial forms of PD (Newmann et al., 2002). Recent evidence suggests that phosphorylation of alpha-synuclein at ser129 plays a role in the formation of inclusions in PD (Smith et al., 2005; Masliah et al., 2005).

products description :

Affinity purified rabbit polyclonal antibody

products references :

Okochi M, Walter J, Koyama A, Nakajo S, Baba M, Iwatsubo T, Meijer L, Kahle PJ, Haass C (2000) Constitutive phosphorylation of the Parkinson's disease associated alphasynuclein. J Biol Chem Jan 7;275(1): 390-7. Neumann M, Kahle PJ, Giasson BI, Ozmen L, Borroni E, Spooren W, Muller V, Odoy S, Fujiwara H, Hasegawa M, Iwatsubo T, Trojanowski JQ, Kretzschmar HA, Haass C (2002) Misfolded proteinase k resistant hyperphosphorylated alpha-synuclein in aged transgenic mice with locomotor deterioration and in human alpha synucleinopathies. J Clin Invest Nov; 110(10):1403-5. Smith WW, Margolis RL, Li X, Troncoso JC, Lee MK, Dawson VL, Dawson TM, Iwatsubo T, Ross CA (2005) Alpha-synuclein phosphorylation enhances eosinophilic cytoplasmic inclusion formation in SH-SY5Y Cells. J Neurosci June 8; 25(23):5544-5552. Masliah E, Rockenstein E, Adame A, Alford M, Crews L, Hashimoto M, Suebert P, Lee M, Goldstein J, Chilcote T, Games D, Schenk D (2005) Effects of alpha-synuclein immunization in a mouse model of Parkinson's disease. Neuron Jun 16;46(6):857-868.

ncbi gi num :

9507125

ncbi acc num :

NP_062042.1

ncbi gb acc num :

NM_019169.2

uniprot acc num :

P37377

ncbi mol weight :

ncbi pathways :

Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Amyloids Pathway 936191!!Disease Pathway 936186!!EGFR1 Signaling Pathway 198427!!Parkinson's Disease Pathway 83490

ncbi summary :

may play a role in regulation of synaptic vesicle biogenesis and organization; may be involved in neurotransmission [RGD, Feb 2006]

uniprot summary :

Function: May be involved in the regulation of dopamine release and transport. Subunit structure: Interacts with UCHL1. Ref.7. Subcellular location: Cytoplasm . By similarity. Tissue specificity: Found only in brain (hippocampus, brainstem and cortex). Specifically expressed in neuronal cell bodies and synapses. Post-translational modification: Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress . By similarity.Ubiquitinated. The predominant conjugate is the diubiquitinated form. Ref.7Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure . By similarity. Sequence similarities: Belongs to the synuclein family.

size :

0.1 ml

price :

270 USD