antibody

Anti-14-3-3 Protein

MBS502002

0.1 mL

345 USD

polyclonal

rabbit

nonconjugated

Antibody

Anti-14-3-3 Protein

14-3-3 Protein

14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; 14-3-3 protein beta/alpha; KCIP-1; 14-3-3 protein beta-subtype; prepronerve growth factor RNH-1; protein kinase C inhibitor protein 1; tyrosine 3/tryptophan 5 -monooxygenase activation protein beta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma; 3-monooxygenase/tryptophan 5 monooxygenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5 monooxgenase activation protein, beta polypeptide; tyrosine 3-monooxgenase/tryptophan 5-monooxgenase activation protein, beta polypeptide; tryosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta; Prepronerve growth factor RNH-1; Protein kinase C inhibitor protein 1

YWHAB

Ywhab; Ywhab; KCIP-1

1433B_RAT

Polyclonal

Rabbit

Rat

246

Specific for the ~29k 14-3-3 protein. Immunolabeling of the 14-3-3 protein band is completely blocked by pre-adsorption of the antibody with the peptide that was used to generate the antibody.

Affinity Purified (Prepared from rabbit serum by affinity purification via chromatography on an affinity column prepared with the N-terminal peptide used as antigen.)

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Store at -20 degree C; stable for at least one year.

Western Blot (WB)

Quality Control: Western blots performed on each lot. WB: 1:1000. IHC: Not recommended for use in paraffin sections

Antigen: Peptide corresponding to amino acid residues from the N-terminal region of the rat 14-3-3 protein, conjugated to keyhole limpet hemocyanin (KLH). Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH. Immunogen Species: Rat

Reactivity Assumed Based on 100% Sequence Homology: Bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primates, Xenopus and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen. Biological Significance: 14-3-3 proteins are a family of highly conserved proteins that appear to have multiple roles in cell signaling (Bridges and Moorhead, 2005). The proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different neurological disorders (Berg et al., 2003). 14-3-3 proteins bind protein ligands that are typically phosphorylated on serine or threonine residues and regulate the functions of these binding partners by a number of different mechanisms (Silhan et al., 2004; Dougherty and Morrison, 2004). The 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking.

Affinity purified rabbit polyclonal antibody

Berg D, Holzmann C, Riess O (2003) 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 4:752-762. Bridges D, Moorhead GB (2005) 14-3-3 Proteins: a number of functions for a numbered protein. Sci STKE 2005:re10. Dougherty MK, Morrison DK (2004) Unlocking the code of 14-3-3. J Cell Sci 117:1875-1884. Silhan J, Obsilova V, Vecer J, Herman P, Sulc M, Teisinger J, Obsil T (2004) 14-3-3 Protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding. J Biol Chem 279:49113-49119. Irina Surgucheva, Valery I. Shestopalov, and Andrei Surguchov (2008) Effect of -Synuclein Silencing on Apoptotic Pathways in Retinal Ganglion Cells J. Biol. Chem., 283: 36377 - 36385.

9507243

NP_062250.1

NM_019377.1

P35213

29

ARMS-mediated Activation Pathway (936054); Activation Of BAD And Translocation To Mitochondria Pathway (935932); Activation Of BH3-only Proteins Pathway (935931); Adaptive Immune System Pathway (936651); Alpha6-Beta4 Integrin Signaling Pathway (198503); Apoptosis Pathway (935920); Axon Guidance Pathway (936266); Calcium Regulation In The Cardiac Cell Pathway (198495); Cell Cycle Pathway (83446); Cell Cycle Pathway (463)

member of the 14-3-3 protein family; may mediate signal transduction by binding to phosphoserine-containing proteins; may play a role in cell cycle regulation [RGD, Feb 2006]

Function: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2 . By similarity. Subunit structure: Homodimer. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1 (phosphorylated form); the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2, YAP1 and PRKCE (phosphorylated form). Interacts with SAMSN1 . By similarity. Interacts with PKA-phosphorylated AANAT. Interacts with the phosphorylated (by AKT1) form of SRPK2 . By similarity. Ref.5. Subcellular location: Cytoplasm. Melanosome . By similarity. Post-translational modification: The alpha, brain-specific form differs from the beta form in being phosphorylated . By similarity. Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion . By similarity.Isoform Short contains a N-acetylmethionine at position 1 . By similarity. Sequence similarities: Belongs to the 14-3-3 family.

0.1 mL

345 USD