catalog number :
MBS500034
products full name :
Anti-Nuclei
products short name :
Nuclei
products gene name :
marker for human cells in xenographic research
specificity :
Specific for human nuclei. Stains nuclei of all human cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. By immunoprecipitation the antibody reacts with an 80 kDa and 70 kDa band. The antibody works on acetone fixed and paraformaldehyde fixed cells. It is suggested that you permeabilize the cells with 0.1-0.4% Triton X100. The antibody also works for immunohistochemistry on paraformaldehyde fixed cryostat sections.
purity :
Total IgG fraction (culture supernatant.)
form :
100 ul culture supernatant
storage stability :
Store at -20 degree C in undiluted aliquots; stable for up to one year after date of receipt. Avoid freeze/thaw cycles.
tested application :
Immunofluorescence (IF), Immunohistochemistry (IHC)
app notes :
Quality Control: Immunofluorescence performed on each lot. IHC: 1: 100. IF: 1:100
other info1 :
Immunogen Information: Human cell homogenate. Immunogen Species: Human. Packaging: 100ul culture supernantant. Adequate amount of material to conduct 10- mini Western Blots.
other info2 :
Species Reactivity Note: The antibody has been directly tested for reactivity in human. This antibody does not react with mouse or rat nuclei. Biological Significance: This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells, with no reactivity to rat or mouse cells. Packaging: 100ul culture supernantant. Adequate amount of material to conduct 10 mini Western Blot.
products description :
Mouse monoclonal antibody
products references :
Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9.