Anti-Synaptobrevin (VAMP) | MyBioSource MBS500033 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Anti-Synaptobrevin (VAMP)

catalog :

MBS500033

quantity :

0.1 ml

price :

270 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

SP10

reactivity :

human, mouse

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS500033

products type :

Antibody

products full name :

Anti-Synaptobrevin (VAMP)

products short name :

Synaptobrevin (VAMP)

other names :

vesicle-associated membrane protein 1 isoform 1; Vesicle-associated membrane protein 1; vesicle-associated membrane protein 1; synaptobrevin 1; synaptobrevin-1; vesicle-associated membrane protein 1 (synaptobrevin 1); Synaptobrevin-1

products gene name :

VAMP1/2

other gene names :

VAMP1; VAMP1; SYB1; VAMP-1; SYB1; VAMP-1

uniprot entry name :

VAMP1_HUMAN

clonality :

Monoclonal

isotype :

IgM

clone :

SP10

host :

Mouse

reactivity :

Bovine, human, mouse, pig, rat, rabbit, hamster

sequence length :

118

specificity :

The antibody is specific for the ~16kDa synaptobrevin protein in Western blots of rat brain lysate. The antibody has also been demonstrated to work in immunohistochemistry on formalin fixed, vibratome sections, but does not work on paraffin sections.

purity :

IgM Purified (Purified culture supernatant.)

form :

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

storage stability :

Store at -20 degree C; stable for at least one year.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Quality Control: Western blots performed on each lot. WB: 1:1,000. IHC: 1:100

other info1 :

Antigen: Synaptic immunoprecipitate (crude) from human brain. Immunogen Information: Synaptic immunoprecipitate (crude) from human brain. Immunogen Species: Human

other info2 :

Species Reactivity Note: The antibody has been directly tested for reactivity in human, pig, hamster, rat, rabbit, mouse, and bovine. Biological Significance: Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer's disease (Sze et al., 2000).

products description :

Mouse monoclonal antibody

products references :

Deák F, Schoch S, Liu X, Südhof TC, Kavalali ET (2004) Synaptobrevin is essential for fast synaptic-vesicle endocytosis. Nat Cell Biol. 2004 Nov;6(11):1102-8. Sze CI, Bi H, Kleinschmidt-DeMasters BK, Filley CM, Martin LJ (2000) Selective regional loss of exocytotic presynaptic vesicle proteins in Alzheimer's disease brains. J Neurol Sci.175(2):81-90. Honer, W. G., Hu, L. & Davies, P. Human synaptic proteins with a heterogeneous distribution in cerebellum and visual cortex (1993). Brain Research 609, 9-20.

ncbi gi num :

7657673

ncbi acc num :

NP_055046.1

ncbi gb acc num :

NM_014231.3

uniprot acc num :

P23763

ncbi mol weight :

ncbi pathways :

BoNT Light Chain Types B, D, And F Cleave VAMP/Synaptobrevin Pathway 105737!!Botulinum Neurotoxicity Pathway 105734!!Disease Pathway 530764!!Neuronal System Pathway 106513!!Proteolytic Cleavage Of SNARE Complex Proteins Pathway 105736!!SNARE Interactions In Vesicular Transport Pathway 83057!!SNARE Interactions In Vesicular Transport Pathway 467

ncbi summary :

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. VAMP1 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Multiple alternative splice variants that encode proteins with alternative carboxy ends have been described, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Subunit structure: Interacts with VAPA and VAPB. Subcellular location: Isoform 1: Cytoplasmic vesicle secretory vesicle synaptic vesicle membrane; Single-pass type IV membrane protein . By similarity. Cell junction synapse synaptosome . By similarity Ref.2. Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein . By similarity. Cell junction synapse synaptosome . By similarity Ref.2. Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein Ref.2. Tissue specificity: Nervous system, skeletal muscle and adipose tissue. Sequence similarities: Belongs to the synaptobrevin family.Contains 1 v-SNARE coiled-coil homology domain.

size :

0.1 ml

price :

270 USD

more info or order :

MyBioSource product webpage